Association of the Serotonin Receptor 3E Gene as a Functional Variant in the MicroRNA-510 Target Site with Diarrhea Predominant Irritable Bowel Syndrome in Chinese Women

Journal of Neurogastroenterology and Motility
Yu ZhangWeijuan Gong

Abstract

The functional variant (rs56109847) in the 3'-untranslated regions (3'-UTR) of the serotonin receptor 3E (HTR3E) gene is associated with female diarrhea predominant irritable bowel syndrome (IBS-D) in British populations. However, the relationship of the polymorphism both to HTR3E expression in the intestine and to the occurrence of Chinese functional gastrointestinal disorders has yet to be examined. Polymerase chain reaction amplification and restriction fragment length polymorphism analyses were employed to detect polymorphisms among Chinese Han women, particularly 107 patients with IBS-D, 99 patients with functional dyspepsia (FD), 115 patients with mixed IBS and 69 patients with IBS-D + FD. We also assessed microRNA-510 (miR-510) and HTR3Eexpression in human colonic mucosal tissues with immunohistochemistry and other methods. Dual-luciferase reporter assays were conducted to examine the binding ability of miR-510 and HTR3E 3'-UTR. Genotyping data showed the variant rs56109847 was significantly associated with IBS-D, but not with FD, mixed-IBS, or FD + IBS-D. HTR3E was abundantly expressed around the colonic mucosal glands but less expressed in the stroma. miR-510 expression decreased, whereas HTR3E expression increased in ...Continue Reading

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Citations

Apr 2, 2016·Journal of Neurogastroenterology and Motility·Jung Ho Park
Oct 21, 2016·Human Mutation·Jacopo CelliBeate Niesler
May 12, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Cristina MartínezBeate Niesler
Jun 17, 2020·World Journal of Gastroenterology : WJG·Qi-Yun XiaoGui-Jun Fei
Jun 9, 2018·World Journal of Gastroenterology : WJG·Niranga Manjuri Devanarayana, Shaman Rajindrajith
Jun 11, 2019·Gastroenterology Research and Practice·Lijun DuHui Ren

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Methods Mentioned

BETA
biopsy
PCR
electrophoresis
transfection

Software Mentioned

Statistical Program for Social Sciences

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