Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

FEBS Letters
Eun Kyung ShimJong Ran Lee

Abstract

To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

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Citations

Feb 24, 2006·Nature Reviews. Immunology·Gary A KoretzkyMichael A Silverman
Nov 6, 2013·The Journal of Experimental Medicine·Audrey Le Floc'hMorgan Huse
Jul 9, 2010·Cold Spring Harbor Perspectives in Biology·Lakshmi BalagopalanConnie L Sommers
Feb 16, 2006·Molecular and Cellular Biology·Michael A SilvermanGary A Koretzky
Oct 31, 2014·Cellular and Molecular Life Sciences : CMLS·Audrey Le Floc'h, Morgan Huse
Sep 2, 2008·Immunological Reviews·Jodi L BucklerLaurence A Turka
Sep 14, 2013·European Journal of Immunology·Nadia LadyginaWai-Ping Fung-Leung
Mar 25, 2009·Biochimica Et Biophysica Acta·Robert L Geahlen
Feb 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eun Kyung ShimJong Ran Lee
Jun 15, 2010·European Journal of Immunology·Seung Hee JungJong Ran Lee
May 20, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min Jung LeeNora A Barrett
Mar 9, 2018·Frontiers in Immunology·Anna KabanovaCosima Tatiana Baldari
Jun 24, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seung Hee JungJong Ran Lee

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