Association of TRAF1, TRAF2, and TRAF3 with an Epstein-Barr virus LMP1 domain important for B-lymphocyte transformation: role in NF-kappaB activation.

Molecular and Cellular Biology
O DevergneG Mosialos

Abstract

The Epstein-Barr virus (EBV) transforming protein LMP1 appears to be a constitutively activated tumor necrosis factor receptor (TNFR) on the basis of an intrinsic ability to aggregate in the plasma membrane and an association of its cytoplasmic carboxyl terminus (CT) with TNFR-associated factors (TRAFs). We now show that in EBV-transformed B lymphocytes most of TRAF1 or TRAF3 and 5% of TRAF2 are associated with LMP1 and that most of LMP1 is associated with TRAF1 or TRAF3. TRAF1, TRAF2, and TRAF3 bind to a single site in the LMP1 CT corresponding to amino acids (aa) 199 to 214, within a domain which is important for B-lymphocyte growth transformation (aa 187 to 231). Further deletional and alanine mutagenesis analyses and comparison with TRAF binding sequences in CD40, in CD30, and in the LMP1 of other lymphycryptoviruses provide the first evidence that PXQXT/S is a core TRAF binding motif. The negative effects of point mutations in the LMP1(1-231) core TRAF binding motif on TRAF binding and NF-kappaB activation genetically link the TRAFs to LMP1(1-231)-mediated NF-kappaB activation. NF-kappaB activation by LMP1(1-231) is likely to be mediated by TRAF1/TRAF2 heteroaggregates since TRAF1 is unique among the TRAFs in coactivating ...Continue Reading

References

Sep 1, 1985·Journal of Virology·K P MannD A Thorley-Lawson
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K HennessyE Kieff
Jan 1, 1994·Annual Review of Immunology·J BanchereauS Saeland
Mar 10, 1995·Science·G ChengD Baltimore
Mar 1, 1993·The Journal of Experimental Medicine·Z EstrovB B Aggarwal
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M KayeE Kieff
Apr 15, 1996·Genes & Development·G Cheng, D Baltimore

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Citations

Jun 1, 2000·Leukemia & Lymphoma·J Teruya-FeldsteinE S Jaffe
Feb 20, 2007·Oral Oncology·Mariana Villarroel DorregoA William Barrett
Apr 17, 2004·British Journal of Haematology·Maher K GandhiRajiv Khanna
Oct 25, 2011·Immunological Reviews·Joanne M HildebrandGail A Bishop
Feb 23, 2012·Pediatric Transplantation·Olivia HattonCarlos O Esquivel
Nov 18, 2008·Seminars in Cancer Biology·J M Middeldorp, D M Pegtel
Dec 24, 2005·Pathology·Maher K Gandhi, Rajiv Khanna
Jun 21, 2002·The Journal of General Virology·Stewart Hay, George Kannourakis
Feb 13, 2001·The Journal of General Virology·S HörB Biesinger
Feb 18, 2010·Biochimie·Imen Najjar, Remi Fagard
Oct 3, 2015·The Journal of Microbiology·Sang Yong KimYoon-Jae Song
Mar 6, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jung-Eun KimYoon-Jae Song
Sep 1, 2005·Virology·Sang-Myeong Lee, Steven B Kleiboeker
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Nov 14, 2007·PLoS Pathogens·Kathy H Y ShairNancy Raab-Traub
Jan 19, 2016·Protein Science : a Publication of the Protein Society·Glenna Wink Foight, Amy E Keating
May 9, 2014·PLoS Pathogens·Maha SioudaMassimo Tommasino
Jul 6, 2016·Annals of Hematology·Myriam Ben DhiabMounir Trimeche
Jan 18, 2014·Cytokine & Growth Factor Reviews·Zuoan YiGail A Bishop
Jun 8, 1999·Annual Review of Immunology·D WallachM P Boldin
Dec 23, 2014·Cytokine & Growth Factor Reviews·Zulema Antonia PercarioElisabetta Affabris

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