Association of transport-defective light chains with immunoglobulin heavy chain binding protein

Molecular Immunology
J MaLinda M Hendershot

Abstract

Immunoglobulin light chains are usually secreted from cells when they are synthesized alone or in molar excess of heavy chains, but, there have been reports of nonsecreted light chains. We wished to determine whether immunoglobulin heavy chain binding protein (BiP), which blocks the transport of free heavy chains, might be responsible for the lack of secretion of some light chains. In two murine lymphoid cell lines that synthesize but do not secrete immunoglobulin light chains, the free light chain polymers were found bound to BiP. Examination of 20 other cell lines and hybridomas failed to disclose any cells synthesizing free or excess light chains that associated with BiP, in all cases the free light chains were secreted as dimers. Despite their association with BiP and their blocked secretion, the aberrant light chains could combine with heavy chains and could be secreted as intact Ig molecules. Thus, while light chains do not usually express signals which allow them to bind to BiP, it appears that such signals can be expressed on certain light chains, resulting in their combination with BiP and blocked secretion. When single chain mutant cell lines are isolated from parental lines producing both heavy and light chains, they...Continue Reading

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Citations

May 5, 2005·Molecular Immunology·Andreas F Kolb, C Bruce A Whitelaw
Jun 11, 2002·Journal of Molecular Biology·Gerhard KnarrJohannes Buchner
Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Colleen M DoyleEline T Luning Prak
Nov 29, 2007·Molecular Cell·Yuki Okuda-Shimizu, Linda M Hendershot
Jul 30, 2005·Archives of Biochemistry and Biophysics·Peter J SimonDaniel A Soltis
Oct 24, 2003·Biotechnology and Bioengineering·Simone M SchatzFriedrich Scheiflinger
May 25, 2004·Journal of Leukocyte Biology·Catherine DecourtMichel Cogné
Feb 12, 2005·The Journal of Biological Chemistry·Yechiel ElkabetzShoshana Bar-Nun
Apr 20, 2004·Blood·Daniel CoriuAlan Solomon
Nov 17, 1995·The Journal of Biological Chemistry·G KnarrJ Buchner
Oct 9, 1999·The Journal of Biological Chemistry·G KnarrJ Buchner
Jun 11, 2021·Biotechnology and Bioengineering·Cai GuoRené Hubert

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