Association of urinary macromolecules with calcium oxalate crystals induced in vitro in normal human and rat urine

Urological Research
F AtmaniS R Khan

Abstract

This study was undertaken to identify proteins which are found associated with calcium oxalate crystals induced in vitro in normal human and rat urine. Crystallization was initiated by adding sodium oxalate individually to each urine sample without centrifugation and filtration. Crystals were collected and analyzed by scanning electron microscopy and X-ray diffraction. Crystal matrix proteins (CMPs) were obtained by demineralization of the crystals with ethylenediaminetetra-acetic acid (EDTA) and analyzed by western blotting technique for immunological identification. Crystals produced in human urine were found to be a mixture of calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) while those produced in rat urine were exclusively COD. CMPs extracted from crystals in human urine comprised, in addition to prothrombin-related proteins, osteopontin and albumin. However, CMPs extracted from crystals in rat urine contained only osteopontin and albumin. Prothrombin-related proteins were found only in trace amounts. In a separate experiment, rat urine samples were supplemented with COM before inducing crystallization. Similar results were observed showing that CMP contained osteopontin, albumin and trace amounts of p...Continue Reading

References

Jun 1, 1977·The Journal of Clinical Investigation·J B LianP M Gallop
Sep 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E M WorcesterD L Lewand
Apr 30, 1992·Biochemical and Biophysical Research Communications·K KohriT Kurita
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShiragaE G Neilson
Dec 15, 1985·The Biochemical Journal·A Franzén, D Heinegård
Nov 1, 1968·The American Journal of Medicine·W H Boyce
Jan 1, 1981·Calcified Tissue International·M A WarpehoskiE P Goldberg
Sep 30, 1993·Biochemical and Biophysical Research Communications·A M StapletonR L Ryall
Jan 1, 1993·Urological Research·P N ShevockR L Hackett
Dec 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M D McKeeS R Khan
Sep 1, 1956·The Journal of Urology·W H BOYCE, F K GARVEY
Oct 1, 1961·The Journal of Urology·B FINLAYSONE J STEWART

❮ Previous
Next ❯

Citations

Aug 18, 2005·Kidney International·Hilary J VernonAmrik Sahota
Oct 23, 2004·Nephron. Physiology·Jack G KleinmanJeremy Hughes
Oct 24, 2013·BioMed Research International·Kanu Priya AggarwalChanderdeep Tandon
Mar 23, 2005·QJM : Monthly Journal of the Association of Physicians·R W E Watts
Feb 19, 1998·British Journal of Urology·C J DawsonR L Ryall
Apr 3, 2009·American Journal of Physiology. Renal Physiology·Putita SaetunVisith Thongboonkerd
Nov 18, 1998·Microbial Pathogenesis·S DaskalovaA Ivanov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.