Association of Vibrio parahaemolyticus thermostable direct hemolysin with lipid rafts is essential for cytotoxicity but not hemolytic activity.

Infection and Immunity
Shigeaki MatsudaTetsuya Iida

Abstract

Thermostable direct hemolysin (TDH), a major virulence factor of Vibrio parahaemolyticus, induces cytotoxicity in cultured cells. However, the mechanism of TDH's cytotoxic effect including its target molecules on the plasma membrane of eukaryotic cells remains unclear. In this study, we identified the role of lipid rafts, cholesterol- and sphingolipid-enriched microdomains, in TDH cytotoxicity. Treatment of cells with methyl-beta-cyclodextrin (MbetaCD), a raft-disrupting agent, inhibited TDH cytotoxicity. TDH was associated with detergent-resistant membranes (DRMs), and MbetaCD eliminated this association. In contrast, there was no such association between a nontoxic TDH mutant and DRMs. The disruption of lipid rafts neither affected hemolysis nor inhibited Ca(2+) influx into HeLa cells induced by TDH. These findings indicate that the cytotoxicity but not the hemolytic activity of TDH is dependent on lipid rafts. The exogenous and endogenous depletion of cellular sphingomyelin also prevented TDH cytotoxicity, but a direct interaction between TDH and sphingomyelin was not detected with either a lipid overlay assay or a liposome absorption test. Treatment with sphingomyelinase (SMase) at 100 mU/ml disrupted the association of TDH...Continue Reading

References

Nov 1, 1992·Canadian Journal of Microbiology·T HondaJ Kim
Nov 1, 1984·Analytical Biochemistry·K Nakamura, S Handa
Jan 1, 1980·Annual Review of Microbiology·P A BlakeD G Hollis
May 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·M YohT Honda
Jun 5, 1997·Nature·K Simons, E Ikonen
Mar 28, 1998·The Journal of Biological Chemistry·A YamajiM Umeda
Feb 7, 2001·Trends in Microbiology·L AbramiF G van der Goot
Dec 5, 2000·Current Biology : CB·C M RosenbergerB B Finlay
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·I WalevS Bhakdi
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Jul 17, 2001·Current Opinion in Cell Biology·E Ikonen
Jul 18, 2002·The Journal of Biological Chemistry·Wayne SchrawTimothy L Cover
Jan 29, 2003·The Journal of Cell Biology·Laurence AbramiF Gisou van der Goot
Jul 24, 2003·Nature Reviews. Immunology·Santos MañesCarlos Martínez-A
Jul 30, 2003·The Journal of Cell Biology·Eric C Lai
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Dec 20, 2003·Nature·Kentaro HanadaMasahiro Nishijima
Dec 26, 2003·Biophysical Journal·Reiko IshitsukaToshihide Kobayashi
Mar 11, 2004·Cellular Microbiology·Philipp A LangStephan M Huber
Mar 24, 2004·Current Opinion in Microbiology·Frank LafontF Gisou van der Goot
Apr 6, 2005·Journal of Biochemistry·Reiko IshitsukaToshihide Kobayashi
Apr 21, 2005·The Journal of Biological Chemistry·Etsuko KiyokawaToshihide Kobayashi
Jul 6, 2005·Trends in Biochemical Sciences·Dov LichtenbergHeiko Heerklotz

❮ Previous
Next ❯

Citations

Apr 27, 2012·Journal of Clinical Microbiology·Jessica L JonesAngelo DePaola
May 26, 2010·PloS One·Panagiotis PapatheodorouKlaus Aktories
Jun 5, 2013·Archives of Virology·Martín Ramírez-OrozcoSergio F Martínez-Díaz
Feb 26, 2013·Current Opinion in Microbiology·Lingling Zhang, Kim Orth
Sep 25, 2015·Scientific Reports·Ming Liu, Sheng Chen
Feb 24, 2015·Immunological Reviews·Olivier NeyrollesMichael Niederweis
Jul 26, 2011·Microbes and Infection·Christopher A BrobergKim Orth
Mar 24, 2015·Frontiers in Microbiology·Rongzhi WangShihua Wang
Jul 19, 2016·Fish & Shellfish Immunology·Wan PengDan-Qi Lu
Dec 15, 2012·The Journal of Veterinary Medical Science·Hiroyuki SugiyamaNobuyuki Susa
Dec 19, 2019·Microbiology and Immunology·Shigeaki MatsudaToshio Kodama
May 10, 2017·Frontiers in Microbiology·Vengadesh LetchumananLearn-Han Lee
May 31, 2018·Microbial Genomics·Nicholas Petronella, Jennifer Ronholm
Jan 5, 2021·Nihon saikingaku zasshi. Japanese journal of bacteriology·Shigeaki Matsuda
Apr 1, 2021·Journal of Applied Microbiology·S AliZ H Mahmud
Aug 10, 2021·Frontiers in Molecular Biosciences·Pratima Verma, Kausik Chattopadhyay
Aug 15, 2021·Environmental Microbiology·Kyle D BrumfieldRita R Colwell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.