Associations between milk performance traits in Holstein cows and 16 candidate SNPs identified by arrayed primer extension (APEX) microarray

Animal Biotechnology
Stanisław KaminskiR Mägi

Abstract

An oligonucleotide microarray-which allows for parallel genotyping of many SNPs in genes involved in cow milk protein biosynthesis-was used to identify which of the 16 candidate SNPs are associated with milk performance traits in Holstein cows. Four hundred cows were genotyped by the developed and validated microarray. Significant associations were found between four single SNPs, namely DGAT1 (acyloCoA:diacylglycerol acyltransferase), LTF (lactoferrin), CSN3 (kappa-casein), and GHR (growth hormone receptor) and with fat and protein yield and percentage. Many significant associations between combined genotypes (two SNPs) and milk performance traits were found. The associations between the combined genotypes DGAT1/LTF and DGAT1/LEPTIN analyzed traits are presented as examples. The microarray based on APEX (Arrayed Primer Extension) is a fast and reliable method for multiple SNP analysis of potential application in marker-assisted selection. After further development, the chip may prospectively be used for dairy cattle paternity analysis and evolutionary studies.

References

Jan 1, 1996·Human Mutation·J M ShumakerC T Caskey
Aug 21, 2003·Journal of Animal Science·G ThallerR Fries

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Citations

Aug 2, 2007·Journal of Applied Genetics·Stanisław KamińskiElzbieta Kostyra
Oct 7, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Eveline M Ibeagha-AwemuXin Zhao
Oct 22, 2013·Animal Reproduction Science·E M HomerP C Garnsworthy
Jun 21, 2008·Journal of Dairy Science·R C ChebelJ E P Santos
Apr 4, 2015·Environmental Monitoring and Assessment·Mehmuna BegumRamalingam Kirubagaran
May 23, 2014·Nature·Kevin J ForsbergGautam Dantas

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Methods Mentioned

BETA
genotyping
PCR
chip

Software Mentioned

SAS
PicDB
Genorama
Statistica

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