Assorted effects of TGFbeta and chondroitinsulfate on p38 and ERK1/2 activation levels in human articular chondrocytes stimulated with LPS

Osteoarthritis and Cartilage
J HolzmannM Huettinger

Abstract

Inadequate cellular response of chondrocytes to stress frequently terminates in osteoarthritis (OA). Adequate response is fundamentally modulated by concerted cytokine signaling events, directing degradation and synthesis of cartilage on articular surfaces where and whenever necessary. Transforming growth factor (TGF)beta is a prominent mediator in cartilage anabolism, although particular catabolic activities are occasionally reported. Clearly, before the TGFbeta signal gets through to the gene regulatory machinery, cross talk with modulators occurs. We tested the hypothesis whether chondroitinsulfate (CS) modulates cell signaling. TGFbeta and/or soluble CS was added to human articular chondrocytes (HACs) and activation of p38 and extracellular signal related kinase (ERK)1/2 was determined by immunoblot analysis. Expression levels of mRNA of matrix metalloproteinase (MMP)-2, -3 and -13 were determined by real-time polymerase chain reaction (PCR). No significant effects were observed unless cells were stimulated with lipopolysaccharide (LPS), invigorating catabolic metabolism in chondrocytes. LPS effects, however, were profoundly modulated by TGFbeta, CS and both applied in combination. Most prominent, the silencing of p38 stres...Continue Reading

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Citations

Aug 21, 2013·Current Rheumatology Reports·Yves Henrotin, Cécile Lambert
Dec 1, 2010·Therapeutic Advances in Musculoskeletal Disease·Yves HenrotinCecile Lambert
Nov 15, 2008·Journal of Cellular Biochemistry·Giuseppe M CampoAlberto Calatroni
Jul 22, 2010·Journal of Veterinary Pharmacology and Therapeutics·C VerdeM F Landoni
Sep 15, 2009·Osteoarthritis and Cartilage·N BrandlM Huettinger
Jan 13, 2017·Journal of Tissue Engineering and Regenerative Medicine·Alexander KöwitschThomas Groth
Nov 21, 2018·Current Medicinal Chemistry·Daniel K Afosah, Rami A Al-Horani
Oct 20, 2018·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·A V Fedotova, O P Mironova
Feb 8, 2021·Drug Discovery Today·Sarita Mishra, Munia Ganguli

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