Astaxanthin and withaferin A block paracrine cytokine interactions between UVB-exposed human keratinocytes and human melanocytes via the attenuation of endothelin-1 secretion and its downstream intracellular signaling

Cytokine
Takao NiwanoGenji Imokawa

Abstract

Paracrine interactions between keratinocytes and melanocytes via cytokines play an essential role in regulating pigmentation in epidermal hyperpigmentary disorders. There is an urgent need for a human epidermal model in which melanogenic paracrine interactions between UVB-exposed keratinocytes and melanocytes can be precisely evaluated because human epidermal equivalents consisting of multilayered keratinocytes and melanocytes have significant limitations in this respect. To resolve this challenge, we established a co-culture system with cell inserts using human keratinocytes and human melanocytes that serves as an appropriate new model for UVB-induced hyperpigmentation. Using that new model, we examined the blocking effects of two natural chemicals, astaxanthin and withaferin A, on paracrine cytokine interactions between UVB-exposed keratinocytes and melanocytes and characterized their mechanisms of action. RT-PCR analysis showed that co-culture of human keratinocytes that had been exposed to UVB significantly stimulated human melanocytes to increase their expression of genes encoding microphthalmia-associated transcription factor, tyrosinase and tyrosinase-related protein 1. The catalytic activity of tyrosinase was also incre...Continue Reading

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Citations

May 5, 2016·Medical Hypotheses·Adam M TaylorAaron Bleakley
Jul 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiping DuHui Ni
Apr 15, 2016·Experimental Biology and Medicine·Lijun SunJun Hu
Jun 9, 2020·Experimental and Therapeutic Medicine·Wenhui QianHua Su
Jul 31, 2019·International Journal of Molecular Sciences·Genji Imokawa
Dec 23, 2020·The Journal of Investigative Dermatology·Eleni FitsiouMarco Demaria
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Camille Keisha MahendraBey Hing Goh
May 1, 2021·International Journal of Molecular Sciences·Young In LeeTae-Gyun Kim

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