Astaxanthin binding protein in Atlantic salmon

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Sarah J MatthewsTom A Gill

Abstract

The rubicund pigmentation in salmon and trout flesh is unique and is due to the deposition of dietary carotenoids, astaxanthin and canthaxanthin in the muscle. The present study was undertaken to determine which protein was responsible for pigment binding. Salmon muscle proteins were solubilized by sequential extractions with non-denaturing, low ionic strength aqueous solutions and segregated as such into six different fractions. Approximately 91% of the salmon myofibrillar proteins were solubilized under non-denaturing conditions using a protocol modified from a method described by Krishnamurthy et al. [Krishnamurthy, G., Chang, H.S., Hultin, H.O., Feng, Y., Srinivasan, S., Kelleher. S.D., 1996. Solubility of chicken breast muscle proteins in solutions of low ionic strength. J. Agric. Food Chem. 44: 408-415.] for the dissolution of avian muscle. To our knowledge, this is the first time this solubilization approach has been applied to the study of molecular interactions in myofibrillar proteins. Astaxanthin binding in each fraction was determined using an in vitro binding assay. In addition, SDS-PAGE and quantitative densitometry were used to separate and determine the relative amounts of each of the proteins in the six fractio...Continue Reading

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Citations

Jul 23, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sibel DemirelEmin Ozmert
Mar 6, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N ChimsungJ E Milley
Mar 10, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T Ytrestøyl, B Bjerkeng
Jun 27, 2015·Critical Reviews in Food Science and Nutrition·R M Schweiggert, R Carle
Apr 28, 2016·The Journal of Physiological Sciences : JPS·Toshinori YoshiharaTakao Sugiura
Dec 12, 2018·Journal of the Science of Food and Agriculture·Tania Chacón-OrdóñezRalf Schweiggert
Jul 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mikhail InyushinAstrid Zayas-Santiago
Nov 27, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Made Airanthi K Widjaja-Adhi, Marcin Golczak
May 1, 2009·Biochemistry·Prakash BhosalePaul S Bernstein
Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera

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