Astaxanthin from shrimp efficiently modulates oxidative stress and allied cell death progression in MCF-7 cells treated synergistically with β-carotene and lutein from greens

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Poorigali Raghavendra-Rao SowmyaRangaswamy Lakshminarayana

Abstract

This study investigated the synergistic efficacy of keto-carotenoid astaxanthin (AST, from shrimp) plus hydrocarbon (β-carotene, BC) and hydroxyl (lutein, L) carotenoids (from greens) on molecular events in MCF-7 cells. MCF-7 cells were treated with either of carotenoid (20 μM, AST or BC or L) separately or the mixture of them (an equimolar concentration of carotenoids mixture, CM) or saponified carotenoid extract from shrimp (SSCE) for 48 h and analyzed cellular uptake, cytotoxicity, and apoptosis. The IC50 and combination-index values of AST co-treatment with a lower concentration of BC and L (5 μM) exhibited enhanced cytotoxicity and oxidative stress as compared with individual carotenoids or SSCE. Further, higher cellular uptake/accumulation of AST along with BC and L found to synergistically induce apoptosis through modulation of cyclin D1, p53, Bax and Bcl-2 expressions by arresting cell cycle at G0/G1 phase. Further, CM or SSCE treatments are unlikely to affect proliferation of normal breast epithelial cells (MCF-10A). The results of selective killing of MCF-7 cells demonstrated a greater insight on the synergistic effect of shrimp AST plus BC and L. It is concluded that consumption of shrimp along with green leafy veget...Continue Reading

Citations

Jun 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chi-Hao WangSan-Lang Wang
Aug 30, 2020·Cancers·Lenka KoklesovaPeter Kubatka
Jan 6, 2021·Critical Reviews in Food Science and Nutrition·Saeid MaghsoudiThomas J Webster
Feb 15, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Immacolata FaraoneHaroon Khan
Apr 6, 2021·World Journal of Biological Chemistry·Rama Rao MallaGanji Purnachandra Nagaraju
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yicheng TanZhiwei Liu
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Angel Cabanillas-BojórquezJosé Basilio Heredia

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