Astaxanthin inhibits the reduction of glycolysis during the activation of hepatic stellate cells.

Life Sciences
Minkyung BaeJi-Young Lee

Abstract

Hepatic stellate cells (HSCs) play an essential role in the development of liver fibrosis by producing extracellular matrix proteins, growth factors, and pro-inflammatory and pro-fibrogenic cytokines once activated. We previously demonstrated that astaxanthin (ASTX), a xanthophyll carotenoid, attenuates HSC activation. The objective of this study was to investigate whether there is a difference in glycolysis between quiescent and activated HSCs and the effect of ASTX on glycolysis during HSC activation. Mouse primary HSCs were activated for 7 days in the presence or absence of 25 μM of ASTX. Quiescent HSCs (qHSCs), 1 day after isolation, and activated HSCs (aHSCs) treated with/without ASTX were plated in a Seahorse XF24 cell culture microplate for Glycolysis Stress tests. aHSCs had significantly lower glycolysis, but higher glycolytic capacity, maximum capacity of glycolysis, and non-glycolytic acidification than qHSCs. Importantly, ASTX markedly increased glycolysis during HSC activation with a concomitant increase in lactate formation and secretion. Compared with qHSCs, aHSCs had significantly lower expression of glucose transporter 1, the major glucose transporter in HSCs, and its transcription factor hypoxia-inducible facto...Continue Reading

References

Aug 26, 1998·The American Journal of Pathology·G A RammD H Crawford
Feb 4, 2005·The Journal of Clinical Investigation·Ramón Bataller, David A Brenner
Nov 16, 2010·Annual Review of Pathology·Virginia Hernandez-Gea, Scott L Friedman
Jan 6, 2011·Molecular Nutrition & Food Research·Jian-Ping YuanJiang-Hai Wang
Feb 18, 2011·Journal of Cellular Biochemistry·Jessica Cassavaugh, Karen M Lounsbury
Jan 12, 2012·TheScientificWorldJournal·Haim ShapiroAmiram Ariel
Aug 14, 2012·Gastroenterology·Yuping ChenAnna Mae Diehl
May 3, 2013·The Journal of Clinical Investigation·Chunyue YinDidier Y R Stainier
Feb 26, 2014·Nature Reviews. Immunology·Antonella PellicoroJonathan A Fallowfield
May 7, 2014·The Journal of Biological Chemistry·Mulchand S PatelFrank Jordan
Jan 21, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Sergio DuarteAna J Coito
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Apr 22, 2015·Cell·Dong Chul LeeYoung Il Yeom
Nov 4, 2015·Biochemical and Biophysical Research Communications·Swathi KarthikeyanShanmugasundaram Ganapathy-Kanniappan
Feb 21, 2016·The Journal of Nutritional Biochemistry·Yue YangJi-Young Lee
Apr 1, 2016·PloS One·Shona A MookerjeeMartin D Brand
Dec 5, 2016·The Journal of Nutritional Biochemistry·Yue YangJi-Young Lee
Oct 7, 2017·Cell·Brandon FaubertRalph J DeBerardinis
Oct 19, 2017·Nature·Sheng HuiJoshua D Rabinowitz
Jul 16, 2019·The Journal of Nutritional Biochemistry·Minkyung BaeJi-Young Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.