Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering

Biotechnology for Biofuels
Jin JinYingjin Yuan

Abstract

Astaxanthin is a natural carotenoid pigment with tremendous antioxidant activity and great commercial value. Microbial production of astaxanthin via metabolic engineering has become a promising alternative. Although great efforts have been conducted by tuning the heterologous modules and precursor pools, the astaxanthin yields in these non-carotenogenic microorganisms were still unsatisfactory for commercialization, indicating that in addition to targeted tailoring limited targets guided by rationally metabolic design, combining more globe disturbances in astaxanthin biosynthesis system and uncovering new molecular mechanisms seem to be much more crucial for further development. Since combined metabolic engineering with mutagenesis by screening is a powerful tool to achieve more global variations and even uncover more molecular targets, this study would apply a comprehensive approach integrating heterologous module engineering and mutagenesis by atmospheric and room temperature plasma (ARTP) to promote astaxanthin production in Saccharomyces cerevisiae. Here, compared to the strain with β-carotene hydroxylase (CrtZ) from Alcaligenes sp. strain PC-1, involving new CrtZ from Agrobacterium aurantiacum enhanced astaxanthin yield to...Continue Reading

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Citations

Jul 13, 2019·Journal of Industrial Microbiology & Biotechnology·Yuanqing WuXueming Zhao
Jan 11, 2020·Applied Microbiology and Biotechnology·Qinghui YuGuoquan Hu
Jun 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana AlvesMadalena Pinto
May 28, 2019·Applied Microbiology and Biotechnology·Sandra Moser, Harald Pichler
Mar 20, 2019·Microbial Cell Factories·Chonglong WangSeon-Won Kim
Mar 13, 2019·Microbial Cell Factories·Shuo-Fu Yuan, Hal S Alper
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May 14, 2020·Applied Microbiology and Biotechnology·Congqiang ZhangHeng-Phon Too
Dec 30, 2020·Progress in Lipid Research·Xia WanQian-Chun Deng
Jun 12, 2021·Current Opinion in Biotechnology·Jingyu ZhangXueting Liu
Nov 23, 2021·Frontiers in Bioengineering and Biotechnology·Mengmeng LiuJingrun Ye

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Methods Mentioned

BETA
PCR
gene knocked-out
RNA-seq
gene

Software Mentioned

HiSeq
SAMtools
Htseq
DESeq2

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