Astaxanthin protects against oxidative stress and calcium-induced porcine lens protein degradation

Journal of Agricultural and Food Chemistry
Tzu-Hua WuChao-Chien Hu

Abstract

Astaxanthin (ASTX), a carotenoid with potent antioxidant properties, exists naturally in various plants, algae, and seafoods. In this study, we investigated the in vitro ability of ASTX to protect porcine lens crystallins from oxidative damage by iron-mediated hydroxyl radicals or by calcium ion-activated protease (calpain), in addition to the possible underlying biochemical mechanisms. ASTX (1 mM) was capable of protecting lens crystallins from being oxidized, as measured by changes in tryptophan fluorescence, in the presence of a Fenton reaction solution containing 0.2 mM Fe2+ and 2 mM H2O2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated that beta(high)-crystallin was the most vulnerable protein under these conditions of free radical exposure. The proteolysis of lens crystallins induced by calcium ion-activated calpain was also inhibited by ASTX (0.03-1 mM) as determined by daily measurement of the light-scattering intensity at 405 nm for five consecutive days. ASTX at 1 mM was as potent as a concentration of 0.1 mM calpain inhibitor E64 in protecting the oxidative damage/hydrolysis of porcine crystallins. At a concentration of 1 mM, ASTX provided better protection than the endogenous antioxid...Continue Reading

References

Mar 1, 1975·Experimental Eye Research·G Duncan, A R Bushell
Jan 1, 1987·Bioelectromagnetics·P KramárA W Guy
Dec 1, 1986·Archives of Biochemistry and Biophysics·D GarlandJ Kinoshita
Jul 1, 1971·Experimental Eye Research·E J Sheridan, S Zigman
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W MikiS Konosu
Jan 1, 1981·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·N RosenM Lazar
Oct 1, 1995·Current Eye Research·F Kilic, J R Trevithick
Jun 25, 1999·Biochemical and Biophysical Research Communications·F Y HuangY Ho
Jul 23, 1999·Current Eye Research·Y NakamuraT R Shearer
Sep 29, 1999·The American Journal of Clinical Nutrition·L Chasan-TaberS E Hankinson
Mar 10, 2000·Journal of Epidemiology·S West
Apr 25, 2000·Journal of Agricultural and Food Chemistry·Y M Naguib
May 8, 2000·Nutrition and Cancer·H JyonouchiM D Gross
Nov 7, 2000·International Ophthalmology Clinics·J R Trevithick, K P Mitton
Jul 27, 2001·Archives of Ophthalmology·P F JacquesA Taylor
Sep 22, 2001·Journal of Protein Chemistry·D ManzanaresE A Lissi
Sep 27, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·W Qian, H Shichi
Feb 28, 2002·The American Journal of Clinical Nutrition·Allen TaylorWalter C Willett
May 3, 2003·Trends in Biotechnology·Martin GuerinMiguel Olaizola

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Citations

Dec 1, 2010·The Journal of Physical Chemistry. B·Nikolay E PolyakovLowell D Kispert
Feb 17, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hui ShenBrandon K Harvey
Feb 26, 2016·Journal of Agricultural and Food Chemistry·Jiahn-Haur LiaoTzu-Hua Wu
Sep 21, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P R AugustiT Emanuelli
Aug 7, 2008·Biotechnology Journal·Claudia Grewe, Carola Griehl
Jan 6, 2011·Molecular Nutrition & Food Research·Jian-Ping YuanJiang-Hai Wang
Sep 5, 2013·Colloids and Surfaces. B, Biointerfaces·Pavan SunkireddySubhash C Yadav
Oct 6, 2011·European Journal of Nutrition·Rosemari OttonRita Mattei
Feb 26, 2020·International Journal of Molecular Sciences·Jowita RzajewElzbieta Rebas
Jul 3, 2019·Frontiers in Pharmacology·Devesh TewariGianina Crişan
Sep 17, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jianbin BiJingyao Zhang
Mar 26, 2021·World Journal of Microbiology & Biotechnology·Sijia ZhengQiangang Li
Feb 6, 2009·Chemical Research in Toxicology·Jiahn-Haur LiaoShih-Hsiung Wu
Aug 14, 2013·The Journal of Physical Chemistry. B·Nikolay E PolyakovLowell D Kispert
Sep 29, 2021·Critical Reviews in Food Science and Nutrition·Yunrui CaoJie Xu
Jan 11, 2020·Experimental and Molecular Pathology·Ming YangZhijun Wang

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