Asterosaponin 1, a cytostatic compound from the starfish Culcita novaeguineae, functions by inducing apoptosis in human glioblastoma U87MG cells.

Journal of Neuro-oncology
Guang ChengBo-quan Jin

Abstract

Malignant glioblastoma is one of the most common malignant tumors in the neurological system. Asterosaponin 1, a new cytostatic agent from the starfish Culcita novaeguineae appear to exhibit various biological activities, including antitumor effect, but the function and mechanism of this new agent on glioblastoma cells has not previously been determined. In the present study, we investigated the proliferation change of human glioblastoma U87MG cells exposed to different concentrations (2.5-20.0 microg/ml) of asterosaponin 1 for a certain time. The results showed that asterosaponin 1 significantly suppressed U87MG cell proliferation in a time- and dose-dependent manner (IC50 =4.3 microg/ml). Flow cytometric analysis of DNA in U87MG cells showed that asterosaponin 1 induces the prominent appearance of a sub-G1 peak in the cell cycle suggestive of apoptosis identical with the result of annexin V/PI assay. Furthermore, U87MG cells treatment with asterosaponin 1 resulted in nuclear condensation with apoptotic bodies observed by both fluorescence and electron microscopy. Agarose gel electrophoresis of DNA from asterosaponin 1-treated cells revealed a typical "ladder" consistent with apoptotic DNA fragmentation. Western-blot staining ...Continue Reading

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Citations

Sep 24, 2009·Chemistry & Biodiversity·Hai-Feng TangGuang Cheng
Jan 5, 2018·Journal of Molecular Endocrinology·Francisca CarvalhalAnake Kijjoa
Jan 9, 2008·Apoptosis : an International Journal on Programmed Cell Death·Hong LinPeng Wang
Sep 30, 2008·International Journal of Radiation Biology·Ari MarkkanenJonne Naarala
Aug 28, 2021·Biomedicines·Rodion KhotimchenkoYuri Khotimchenko
Dec 31, 2021·Journal of Biochemical and Molecular Toxicology·Yu-Ye XueGuang Cheng

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