Abstract
Activation of HSC is a pivotal step in hepatic fibrosis. In the activation of HSC, the TGF-β1 plays a key role that can promote the occurrence of hepatic fibrosis by combining with Smad proteins. Astragaloside is the main active component extracted from Radix Astragali that has the effect of antioxidation and hepatoprotection. In the present study, we investigated the mechanism of astragalosides inhibiting hepatic fibrosis in vitro and in vivo. In vitro, astragalosides inhibited the activation of HSC and regulated the expression of MMP-2 and TIMP-2 and reduced the formation of collagen fibers. In vivo, administration of astragalosides decreased the serum ALT, AST, and TBiL in rats by reducing oxidative stress. Astragalosides also attenuated hepatic fibrosis by reducing the concentration of hydroxyproline and inhibiting the formation of collagen fibers. The expressions of TGF-β1, TβR-I, p-Smad 2, and p-Smad 3 were downregulated after astragalosides treatments, while Smad 7 was upregulated compared to the control group. The results indicated that the effect of astragaloside on hepatic fibrosis was related to the inhibition of HSC activation and the modulation of the TGF-β1/Smad signaling pathway.
References
May 1, 1989·The American Review of Respiratory Disease·P PääkköL Ryhänen
Jan 25, 2000·The Journal of Biological Chemistry·S L Friedman
Mar 14, 2003·Biochimica Et Biophysica Acta·Ali AboutweratThomas W Warnes
Sep 5, 2003·Journal of Medicinal Chemistry·J Scott SawyerJonathan M Yingling
May 1, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew Leask, David J Abraham
Oct 4, 2005·Journal of Ethnopharmacology·Shuang-Ying GuiCheng-Yi Wu
Dec 14, 2006·The American Journal of Chinese Medicine·Xiang-Jian ChenJi-Nan Zhang
Sep 29, 2007·Clinical and Experimental Pharmacology & Physiology·Ebtehal El-DemerdashNermeen M Shaffie
Dec 29, 2007·The Journal of Pathology·T A Wynn
May 13, 2008·Gastroenterology·Scott L Friedman
May 29, 2008·Journal of Gastroenterology and Hepatology·Olav A GressnerAxel M Gressner
Oct 12, 2011·Journal of Translational Medicine·Monica MarraPasquale Sperlongano
Oct 12, 2011·Journal of Physiology and Biochemistry·Mohamed A MorsyMagda E Mahmoud
Oct 19, 2011·Cell and Tissue Research·Steven Dooley, Peter ten Dijke
Nov 2, 2011·Therapeutic Advances in Gastroenterology·Michal Cohen-Naftaly, Scott L Friedman
Feb 15, 2012·Journal of Gastroenterology and Hepatology·Keiko IwaisakoTatiana Kisseleva
Apr 10, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Nur-Afidah Mohamed Suhaimi, Lang Zhuo
Sep 27, 2012·Brain Research·Jianjun LiKeliang Xie
Feb 26, 2013·Cytokine·Dingkun GuiNiansong Wang
Aug 2, 2013·American Journal of Physiology. Cell Physiology·Ariane Mallat, Sophie Lotersztajn
Jan 21, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Sergio DuarteAna J Coito
Feb 15, 2015·Gut·Youngmin A LeeScott L Friedman
Apr 29, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Fujun YuJianjian Zheng
Mar 5, 2016·Journal of Gastroenterology·Thomas Greuter, Vijay H Shah
Mar 28, 2016·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Haitao ShiXiaoyan Guo
May 3, 2016·Acta Pharmacologica Sinica·Xue-Mei LiYi-Yang Hu
Nov 30, 2016·Pathology, Research and Practice·Roberta SferraGiovanni Latella
Citations
Oct 2, 2019·Experimental and Therapeutic Medicine·Qian MaoLaiqing Li
Nov 7, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Bo YangFeng Zhang
May 20, 2020·Frontiers in Pharmacology·Yau-Tuen ChanYibin Feng
Dec 10, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Peng-Chun YangShi-Zhu Jiang
Jul 10, 2021·World Journal of Gastroenterology : WJG·Qiu-Ju HanJian Zhang