Abstract
In the present study, we examined the molecular mechanism of astragaloside IV (AS-IV) in high glucose (HG)-induced epithelial-to-mesenchymal transition (EMT) in renal proximal tubular epithelial cells (PTCs). NRK-52E cell viability and apoptosis were determined by the cell counting kit-8 (CCK-8) assay and flow cytometric analysis, respectively. Expressions of E-cadherin, N-cadherin, vimentin, and occludin were measured by Western blot, and those of E-cadherin and N-cadherin were additionally measured by immunofluorescence analysis. Transforming growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) expressions were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. The expressions of Smad2, Smad3, phosphorylated-Smad2 (p-Smad2), and p-Smad3 were measured using Western blot. We found that AS-IV could recover NRK-52E cell viability and inhibit HG-induced cell apoptosis. TGF-β1, α-SMA, Smad2, Smad3, p-Smad2, and p-Smad3 expressions were decreased in the AS-IV-treated groups compared with the HG group. Moreover, the expressions of E-cadherin and occludin were remarkably up-regulated and those of N-cadherin and vimentin were down-regulated in the AS-IV-treated groups compared with the HG gr...Continue Reading
References
Apr 25, 2000·The EMBO Journal·J Massagué, D Wotton
Oct 5, 2001·The American Journal of Pathology·J Yang, Y Liu
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 24, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jin H LiHui Y Lan
May 28, 2003·Biochemical and Biophysical Research Communications·Masaki FujimotoSeijiro Mori
Oct 10, 2003·Nature·Rik Derynck, Ying E Zhang
Dec 16, 2004·American Journal of Physiology. Renal Physiology·Ho Jae HanMary Taub
Aug 18, 2006·Journal of the American Society of Nephrology : JASN·Wendy C BurnsPhillip Kantharidis
Dec 17, 2008·Molecular Biology of the Cell·Sarah K HartenPatrick H Maxwell
Dec 31, 2008·Cell Research·Ying E Zhang
May 30, 2009·Stroke; a Journal of Cerebral Circulation·Wenlan LiuKe Jian Liu
Jun 3, 2009·The Journal of Clinical Investigation·Hervé AcloqueM Angela Nieto
Nov 6, 2009·American Journal of Nephrology·Claire E Hills, Paul E Squires
Dec 18, 2009·American Journal of Physiology. Renal Physiology·Yu Jin Lee, Ho Jae Han
Jan 22, 2010·American Journal of Physiology. Renal Physiology·Li ZhouHui Yao Lan
Jul 2, 2010·Clinical and Experimental Pharmacology & Physiology·Li ZhouLi-Min Lu
Jan 26, 2011·Annual Review of Pathology·Yashpal S KanwarSheldon Chen
Aug 11, 2011·PloS One·Zhi-Mei LvRong Wang
Nov 5, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·David K PackhamHiddo J Lambers Heerspink
Jan 4, 2012·Clinical and Experimental Pharmacology & Physiology·Hui Yao Lan
Feb 22, 2012·Neurochemistry International·Min LiGuo D Gao
Dec 12, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yan-Xu ChangXiu-Mei Gao
Jan 18, 2013·Apoptosis : an International Journal on Programmed Cell Death·Dingkun GuiNiansong Wang
May 31, 2013·Cell Stress & Chaperones·Weiwei QiYong Gu
Sep 5, 2014·Experimental and Therapeutic Medicine·Zhuo ChenSuixin Liu
Dec 3, 2015·Biochemical and Biophysical Research Communications·Quanyou Gong, Fangli Hou
Oct 18, 2016·International Journal of Molecular Medicine·Di CheGuoquan Gao
Nov 20, 2016·Cold Spring Harbor Perspectives in Biology·Ying E Zhang
Nov 9, 2017·Scientific Reports·Xian-Long ZhouLei Li
May 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tiantian CaiXiaohui Zeng
Dec 13, 2018·American Journal of Physiology. Renal Physiology·Wei ZhangQingjuan Liu
Jan 8, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Xiaoli YangXiang He