Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways.

Acta Pharmacologica Sinica
Bo YinMei-Li Lu

Abstract

Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vivo by focusing on calcium-sensing receptor (CaSR) and extracellular signal-regulated kinase 1/2 (ERK1/2). Rat neonatal cardiac myocytes were subjected to a hypoxia/reoxygenation (H/R) procedure in vitro, which significantly decreased the cell viability, increased lactate dehydrogenase (LDH) release, induced cardiomyocyte apoptosis, and increased [Ca2+]i. H/R also increased the expression of CaSR and decreased ERK1/2 phosphorylation levels in H/R-exposed myocytes. Pretreatment with AsIV (60 μmol/L) significantly improved the cell viability and decreased LDH release, attenuated myocyte apoptosis, decreased [Ca2+]i and CaSR expression, and increased the ERK1/2 phosphorylation levels. The protective effects of AsIV against H/R injury were partially inhibited by co-treatment with a CaSR agonist, gadolinium chloride (GdCl3) or with a specific ERK1/2 inhibitor U0126. For in vivo studies, a rat MI/R model was established. ...Continue Reading

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Citations

Jun 14, 2019·Frontiers in Pharmacology·Emiel P C van der VorstYvonne Döring
Sep 29, 2020·Drug Design, Development and Therapy·Yu-Qing TanJun Li
Jan 20, 2021·Medicinal Research Reviews·Hui-Fei SuXue Qiao
Mar 4, 2021·Molecular and Cellular Biochemistry·Li-Fei LuoEn-Sheng Ji

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Methods Mentioned

BETA
coronary artery bypass
electrophoresis

Software Mentioned

Quantity One

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