Astragalus Saponins and Liposome Constitute an Efficacious Adjuvant Formulation for Cancer Vaccines

Cancer Biotherapy & Radiopharmaceuticals
Xiao-Ping ZhangFang-Yu An

Abstract

Cancer vaccines mostly aim to induce cytotoxic T lymphocytes (CTLs) against tumors. An appropriate adjuvant is of fundamental importance for inducing cellular immune response. Since the antigen in particulate form is substantially more immunogenic than soluble form antigen, it is beneficial to interact with antigen-presenting cells membrane to induce robust CD8+ T cell activation following vaccination. Based on previous research, we designed an adjuvant formulation by combining Astragalus saponins, cholesterol, and liposome to incorporate antigen into a particulate delivery system, so as to enhance cellular immune response. Meanwhile, angiogenesis contributes to tumor growth and metastasis, and basic fibroblast growth factor (bFGF) is involved in tumor angiogenesis. Therefore, using lipo-saponins adjuvant formulation and a human recombinant bFGF antigen protein, we tried to induce bFGF-specific CTL response to inhibit tumor angiogenesis to achieve antitumor activity. After five immunizations, the lipo-saponins/bFGF complex elicited robust antibody response and markedly higher amount of interferon-γ in BALB/c mice, resulting in superior antitumor activities. Decreased microvessel density in CD31 immunohistochemistry and the lysi...Continue Reading

References

Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R MontesanoL Orci
Jan 23, 1987·Science·J Folkman, M Klagsbrun
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
Sep 2, 1998·Immunology and Cell Biology·F Morein, K L Bengtsson
Mar 23, 2001·Nature Immunology·J J O'Shea, R Visconti
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·I GurselD M Klinman
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Q WeiW Zhang
Jul 13, 2004·Science·Joseph N Blattman, Philip D Greenberg
Oct 27, 2004·Expert Opinion on Biological Therapy·Joseph G AltinChristopher R Parish
Mar 6, 2007·Vaccine·J C Aguilar, E G Rodríguez
Jun 30, 2009·Vaccine·Xiaoming Song, Songhua Hu
Apr 7, 2010·Science Translational Medicine·Omar A AliDavid J Mooney
Oct 30, 2010·Immunity·Robert L CoffmanRobert A Seder
Jun 12, 2012·Cell·Rakesh K Jain, Peter Carmeliet
Jul 20, 2012·Cancer Letters·Brian M Andersen, John R Ohlfest
Dec 11, 2013·Journal of Cancer Research and Clinical Oncology·Xiuying LiYuquan Wei

❮ Previous
Next ❯

Citations

Sep 11, 2019·Chinese Journal of Integrative Medicine·Yu-Tong Liu, Wen-Liang Lv
Oct 18, 2020·Pharmaceutics·Sahra BashiriMariusz Skwarczynski

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay
enzyme-linked immunosorbent assay

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Cancer Cell Invasion: Nanomedicine

Nanomedicine is a promising alternative for cancer detection and therapy that utilizes nanoparticles, such as liposomes. Nanoparticles can potentially target cancer cell invasion and metastasis. Discover the latest research on Cancer Cell Invasion: Nanomedicine here.