Astrocyte-derived exosomes transfer miR-190b to inhibit oxygen and glucose deprivation-induced autophagy and neuronal apoptosis.

Cell Cycle
Xiaoxi PeiZhilong Zhou

Abstract

Our previous work has verified that astrocytes (AS)-derived exosomes (AS-Exo) inhibited autophagy and ameliorated neuronal damage in experimental ischemic stroke. However, the mechanism of AS-Exo regulation of autophagy remains unclear. The aim of this study was to investigate the regulatory mechanism of AS-Exo on neuronal autophagy. The mouse hippocampal neuronal cell line HT-22 was cultured in oxygen and glucose deprivation (OGD) condition to mimic ischemic injury. The primary astrocytes were used to isolate exosomes. Exosome labeling and uptake by HT-22 cells were observed by confocal laser microscopy. miR-190b expression was determined by qRT-PCR. HT-22 cell vitality and apoptosis were determined by CCK-8 assay and TUNEL staining, respectively. Levels of TNF-α, IL-6 and IL-1β were analyzed by ELISA. Protein levels of apoptosis-related cleaved caspase-3, Bax, Bcl-2 and autophagy-related Beclin-1, LC3-I/II, Atg7, P62 were determined by western blot. A dual-luciferase reporter assay was performed to confirm the direct interaction between miR-190b and Atg7. miR-190b expression in AS-Exo was found to be significantly higher than that in AS. AS-Exo-mediated transfer of miR-190b attenuated OGD-induced neuronal apoptosis via suppre...Continue Reading

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Citations

Nov 27, 2020·Frontiers in Immunology·Lisvaneth MedinaUlrike Kemmerling
Jan 29, 2021·Journal of Cellular and Molecular Medicine·Huifang XingQiang Zhang
Feb 12, 2021·Frontiers in Cell and Developmental Biology·Cristiana PistonoTarja Malm
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Tahereh GharbiGuo-Yuan Yang
Nov 17, 2020·Oxidative Medicine and Cellular Longevity·Xiaoyu WangAnwen Shao
Apr 7, 2021·Pharmacology & Therapeutics·Amir AjoolabadyJun Ren
May 14, 2021·Translational Stroke Research·Yiyang LiYonghua Zhao
Jul 17, 2021·Neurobiology of Disease·Samantha L Reed, Andrew Escayg

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Methods Mentioned

BETA
transmission electron microscopy
transfection
PCR
electrophoresis
Assay
ELISA

Software Mentioned

Targetscan
SPSS
Nanosight

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