Astrocyte-specific transcriptome responses to chronic ethanol consumption

The Pharmacogenomics Journal
Emma K EricksonR Adron Harris

Abstract

Astrocytes play critical roles in central nervous system (CNS) homeostasis and are implicated in the pathogenesis of neurological and psychiatric conditions, including drug dependence. Little is known about the effects of chronic ethanol consumption on astrocyte gene expression. To address this gap in knowledge, we performed transcriptome-wide RNA sequencing of astrocytes isolated from the prefrontal cortex (PFC) of mice following chronic ethanol consumption. Differential expression analysis revealed ethanol-induced changes unique to astrocytes that were not identified in total homogenate preparations. Astrocyte-specific gene expression revealed calcium-related signaling and regulation of extracellular matrix genes as responses to chronic ethanol use. These findings emphasize the importance of investigating expression changes in specific cellular populations to define molecular consequences of chronic ethanol consumption in mammalian brain.

References

Jan 1, 1995·Annual Review of Physiology·A P Braun, H Schulman
Oct 31, 2003·Trends in Neurosciences·Michael V L BennettJuan C Sáez
Apr 20, 2004·Neuroreport·Ana M BlancoConsuelo Guerri
Jan 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Robert PawlakSidney Strickland
Apr 21, 2005·Thrombosis and Haemostasis·Jerry P Melchor, Sidney Strickland
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Nov 18, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jianwen LiuR Dayne Mayfield
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John D CahoyBen A Barres
Mar 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nancy Ann OberheimMaiken Nedergaard
Jan 13, 2011·Alcoholism, Clinical and Experimental Research·Megan K MulliganIgor Ponomarev
Jun 28, 2011·Neurobiology of Aging·Julie E SimpsonUNKNOWN MRC Cognitive Function and Ageing Neuropathology Study Group
Oct 21, 2011·Nature Reviews. Neuroscience·Rita Z Goldstein, Nora D Volkow
Feb 4, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Igor PonomarevR Dayne Mayfield
Feb 22, 2012·Advances in Experimental Medicine and Biology·Renato Frischknecht, Eckart D Gundelfinger
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
May 4, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer L ZamanianBen A Barres
Oct 4, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Moonnoh R LeeDoo-Sup Choi
Mar 28, 2013·Brain and Behavior·Leonardo PignataroNeil L Harrison
May 7, 2013·Nature Medicine·Xiong CaoTian-Ming Gao
Feb 20, 2014·Neural Plasticity·Emma V Jones, David S Bouvier
Mar 25, 2014·Alcoholism, Clinical and Experimental Research·Karen L SmithAnita J Bechtholt
Jun 7, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Cecilia BullM Scott Bowers
Sep 5, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ye ZhangJian Qian Wu
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 3, 2014·Molecular Psychiatry·S P FarrisR D Mayfield
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Feb 11, 2015·Cold Spring Harbor Perspectives in Biology·Won-Suk ChungCagla Eroglu
Mar 25, 2015·PloS One·Elizabeth A Osterndorff-KahanekR Dayne Mayfield
Jun 26, 2015·Nature Neuroscience·Baljit S Khakh, Michael V Sofroniew
Oct 28, 2015·Nature Neuroscience·Won-Suk ChungBeth Stevens
Nov 3, 2015·Nature Neuroscience·Kirti SharmaMikael Simons
Jan 28, 2016·Nature Neuroscience·Narges Bazargani, David Attwell
Apr 22, 2016·Nature Communications·Karpagam SrinivasanDavid V Hansen

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Citations

Oct 8, 2019·Alcoholism, Clinical and Experimental Research·Manuel Sanchez-AlavezBruno Conti
Mar 17, 2020·Addiction Biology·Anna S WardenR Adron Harris
Mar 7, 2020·Human Molecular Genetics·Eric BrennerR Dayne Mayfield
May 29, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·E K EricksonR A Harris
Jul 31, 2019·Scientific Reports·Emma K EricksonR Dayne Mayfield
Jun 21, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jeffrey J Moffat, Dorit Ron
Dec 20, 2019·Alcoholism, Clinical and Experimental Research·Patrick P LoweGyongyi Szabo
Jul 30, 2020·Brain, Behavior, and Immunity·E K GranthamR A Harris
Feb 27, 2019·ACS Chemical Neuroscience·Charmaine EnculescuBenjamin L Schulz

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Datasets Mentioned

BETA
GSE92457

Methods Mentioned

BETA
PCR
Hi-Seq
GTPase

Software Mentioned

Enrichr
FASTQC
HTSeq
GSEA
Picard
Bioconductor package
Cutadapt
DESeq2
Bowtie2
Cufflinks

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