PMID: 9179599Jun 1, 1997Paper

Astrocytes and microglial cells incorporate degenerating fibers following entorhinal lesion: a light, confocal, and electron microscopical study using a phagocytosis-dependent labeling technique

Glia
I Bechmann, R Nitsch

Abstract

Entorhinal lesion leads to anterograde degeneration of perforant path fibers in their main termination zone in the outer molecular layers of the dentate gyrus. Concomitantly, astrocytes become hypertrophic, and microglial cells alter their phenotype, suggesting participation in anterograde degeneration. This study analyzes the involvement of these lesion-induced activated glial cells in the process of phagocytosis of degenerated axonal debris. We established a phagocytosis-dependent labeling technique that allows for direct and simultaneous visualization of both labeled incorporated axonal debris and incorporating glial cells. Stereotaxic application of small crystals of the biotin- and rhodamine-conjugated dextran amine Mini Ruby (MR) into the entorhinal cortex led to strong and stable axonal staining of perforant path axons. Following entorhinal lesion, labeled terminals and fibers condensed and formed small granules. Incorporation of these rhodamine-fluorescent granules resulted in a phagocytosis-dependent cell labeling. During the first 3 days, we were able to identify these cells as microglia by using double-fluorescence and confocal microscopy. The first unequivocally double-labeled astrocytes were found 6 days post lesio...Continue Reading

References

Feb 1, 1979·The Journal of Comparative Neurology·C GallG Lynch
Nov 15, 1991·The Journal of Comparative Neurology·J Gehrmann, G W Kreutzberg
Jan 1, 1989·Journal of Neuroscience Research·M B GraeberG W Kreutzberg
Oct 1, 1988·Experimental Neurology·F H GageD M Armstrong
Aug 1, 1986·The Journal of Experimental Medicine·D GiulianL B Lachman
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Apr 1, 1987·Journal of Neurocytology·W J Streit, G W Kreutzberg
Jan 1, 1985·Annals of the New York Academy of Sciences·C W Cotman, M Nieto-Sampedro
Dec 1, 1985·The Journal of Cell Biology·D Giulian, T J Baker
Oct 1, 1993·Journal of Neuroscience Methods·B Fritzsch

❮ Previous
Next ❯

Citations

Aug 24, 1999·Journal of Neurobiology·G MorF Naftolin
Oct 23, 2002·Journal of Neuroscience Research·Bruce Teter, J Wesson Ashford
Nov 9, 2006·Experimental Brain Research·Leman MutluIngo Bechmann
Dec 15, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Raasay S JonesMarina A Lynch
Mar 3, 2010·Molecular Neurobiology·Lih-Fen LueDouglas G Walker
Feb 26, 2004·Neuroscience·M EijkenboomF J van der Staay
Nov 14, 2000·Journal of Neuroscience Methods·S ThanosN Bodeutsch
Jun 17, 2000·Progress in Neurobiology·C KöbbertS Thanos
May 29, 1998·Progress in Neurobiology·H Aldskogius, E N Kozlova
Jul 22, 1998·Progress in Neurobiology·D A TurnerR Nitsch
Oct 7, 1998·Progress in Neurobiology·M A Cuadros, J Navascués
Mar 15, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M B JensenB Finsen
Jun 19, 2012·Nature Neuroscience·Jennifer S ZiegenfussMarc R Freeman
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Judy V NguyenNicholas Marsh-Armstrong
Nov 22, 2011·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Annette Persson, Elisabet Englund
Feb 16, 2007·The British Journal of Ophthalmology·Jimena F SchmidtM Rosario Hernandez
Aug 27, 2009·PLoS Biology·Won-Suk Chung, Ben A Barres
Sep 19, 2001·Reviews in the Neurosciences·N E Savaskan, R Nitsch
Oct 31, 2002·Reviews in the Neurosciences·Elisabetta Polazzi, Antonio Contestabile
Apr 7, 2010·Journal of Neurosurgery·Tetsuji SekiyaJuichi Ito
Jan 1, 2007·Pakistan Journal of Biological Sciences : PJBS·Mehrdad JahanshahiN Naghdi
Dec 19, 2008·Pakistan Journal of Biological Sciences : PJBS·Jahanshahi MehrdadN Naghdi
Jan 4, 2008·Neuron Glia Biology·Mary A Logan, Marc R Freeman
Feb 18, 2005·The Journal of Cell Biology·Laura BerliocchiPierluigi Nicotera
Aug 22, 2014·Glia·Julia PreisslerAngela Schulz
Jul 17, 2009·Journal of Neuropathology and Experimental Neurology·Helle Hvilsted NielsenBente Finsen
Dec 1, 2006·Clinical Neuroscience Research·Zinaida S VexlerMidori A Yenari

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.