PMID: 8599604Feb 29, 1996Paper

Astrocytes synthesize and secrete prostaglandin D synthetase in vitro

Biochimica Et Biophysica Acta
S GiacomelliC Y Cheng

Abstract

Prostaglandin D synthetase [PGD-S, prostaglandin-H2 D-isomerase, (5Z, 13E)-(15S)-9alpha, 11 alpha-epidioxy-15-hyrdroxyprosta-5,13-dienoate D-isomerase, EC 5,3,99,2], an enzyme that catalyzes the formation of prostaglandin D2, was originally isolated from homogenates of rat brain and spleen and is known to be a membrane-bound enzyme. Subsequent immunohistochemical studies have shown that PGD-S is associated with neurons in the brain of immature rats, whereas in adult rats it is associated with oligodendrocytes. Several recent studies have shown that the beta-trace protein isolated from human cerebrospinal fluid (CSF), the second most abundant protein in human CSF after albumin, is equivalent to PGD-S. In this paper, we report the preparation of a monospecific polyclonal antibody against purified PGD-S isolated from human CSF and the establishment of a specific radioimmunoassay for this protein. Using this radioimmunoassay in conjunction with immunoblot analysis, PGD-S was detected in various biological fluids including serum, aqueous humor, and rete testis fluid. In addition, an antibody prepared against human PGD-S partially cross-reacted with the PGD-S in the rat and ram. Using a monospecific polyclonal antibody prepared again...Continue Reading

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Citations

Jul 15, 2009·Journal of Molecular Neuroscience : MN·Xiaorong XinHanspeter E Killer
Jan 16, 2002·Prostaglandins & Other Lipid Mediators·R BaekerB Schlatterer
Aug 21, 2001·Journal of Neurology, Neurosurgery, and Psychiatry·H F PetereitR Pukrop
Nov 5, 2014·Nature Neuroscience·Amelia TrimarcoCarla Taveggia
May 6, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Hsi-Kung KuoPei-Chang Wu
Nov 5, 2014·Rapid Communications in Mass Spectrometry : RCM·O LardinoisL J Deterding
Sep 20, 2018·International Journal of Molecular Sciences·Dmitry V ChistyakovMarina G Sergeeva

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