Astrocytic Coverage of Dendritic Spines, Dendritic Shafts, and Axonal Boutons in Hippocampal Neuropil

Frontiers in Cellular Neuroscience
Nikolay GavrilovAlexey Semyanov

Abstract

Distal astrocytic processes have a complex morphology, reminiscent of branchlets and leaflets. Astrocytic branchlets are rod-like processes containing mitochondria and endoplasmic reticulum, capable of generating inositol-3-phosphate (IP3)-dependent Ca2+ signals. Leaflets are small and flat processes that protrude from branchlets and fill the space between synapses. Here we use three-dimensional (3D) reconstructions from serial section electron microscopy (EM) of rat CA1 hippocampal neuropil to determine the astrocytic coverage of dendritic spines, shafts and axonal boutons. The distance to the maximum of the astrocyte volume fraction (VF) correlated with the size of the spine when calculated from the center of mass of the postsynaptic density (PSD) or from the edge of the PSD, but not from the spine surface. This suggests that the astrocytic coverage of small and larger spines is similar in hippocampal neuropil. Diffusion simulations showed that such synaptic microenvironment favors glutamate spillover and extrasynaptic receptor activation at smaller spines. We used complexity and entropy measures to characterize astrocytic branchlets and leaflets. The 2D projections of astrocytic branchlets had smaller spatial complexity and ...Continue Reading

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Citations

Apr 1, 2019·CNS Neuroscience & Therapeutics·Bin ZhouRuo-Tian Jiang
May 28, 2019·Molecular Neurobiology·Julianna KardosLászló Héja
Feb 12, 2020·Nature Reviews. Neuroscience·Xinzhu YuBaljit S Khakh
Sep 15, 2019·Pflügers Archiv : European journal of physiology·Alexei VerkhratskyAlexey Semyanov
Sep 27, 2020·Pflügers Archiv : European journal of physiology·Alexei VerkhratskyAlexey Semyanov
Sep 3, 2020·Nature Reviews. Neuroscience·Alexey SemyanovAmit Agarwal
Apr 27, 2019·Frontiers in Synaptic Neuroscience·Silvana Valtcheva, Laurent Venance
Jan 8, 2021·Neurochemical Research·Xiaolu ZhangSinead M O'Donovan
Feb 2, 2021·Neurochemical Research·Menachem Hanani, Alexei Verkhratsky
Sep 24, 2020·Cell Reports·Michel K HerdeChristian Henneberger
Mar 7, 2021·Aging Cell·Alexander PopovAlexey Semyanov
Feb 23, 2021·Journal of Neuroinflammation·Cassandra D GipsonErin E Maher
Feb 27, 2021·Frontiers in Cellular Neuroscience·Amina Aboufares El AlaouiMichele Bellesi
Mar 19, 2021·Frontiers in Cellular Neuroscience·László HéjaJulianna Kardos
May 1, 2021·Frontiers in Cellular Neuroscience·Renae M RyanAnnalisa Scimemi
Jun 27, 2021·Neuropharmacology·Dmitri A Rusakov, Michael G Stewart
Jul 3, 2021·Frontiers in Neuroinformatics·Mickael Zbili, Sylvain Rama
Jun 25, 2021·Frontiers in Cellular Neuroscience·Annamaria LiaGabriele Losi
Aug 10, 2021·Frontiers in Cellular Neuroscience·Leonid P SavtchenkoDmitri A Rusakov
Sep 5, 2021·Trends in Neurosciences·Alexey Semyanov, Alexei Verkhratsky
Nov 3, 2021·Physiological Reviews·Carolin Wichmann, Thomas Kuner

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Methods Mentioned

BETA
electron microscopy

Software Mentioned

Origin
ChanneLab2
Reconstruct
Matlab

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