PMID: 39623Jun 19, 1979

Astroglial and axonal proteins in isolated brain filaments. I. Isolation of the glial fibrillary acidic protein and of an immunologically active cyanogen bromide peptide from brain filament preparations of bovine white matter

Biochimica Et Biophysica Acta
D Dahl, A Bignami

Abstract

The glial fibrillary acidic protein and an immunologically active cyanogen bromide peptide were purified by immunoaffinity chromatography from 8 M urea extracts of brain filament preparations isolated from bovine white matter according to Norton's procedure. The protein accounted for approximately 30% of the total protein in this preparation and for the largest fraction in the 50 000 molecular weight range. The fraction not absorbed to the immuno-Sepharose column reacted with neurofilament antisera by double immunodiffusion. On sodium dodecyl sulfate gel electrophoresis the main bands in the non-adsorbed fraction were at 74 000 daltons and above 100 000. Several bands were seen in the 50 000 molecular weight range. It is concluded that glio- and neurofilaments co-purify together in Norton's procedure and that neurofilaments are probably heterogeneous in polypeptide composition.

References

Nov 1, 1984·Journal of the Neurological Sciences·J G WetmurT S Elizan
Jan 1, 1984·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A BignamiD Dahl
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Jun 10, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qiaozhen YangGang Li
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Citations

Sep 1, 1977·Journal of Ultrastructure Research·W A Day
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S H YenM L Shelanski
Jun 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Bignami, D Dahl
Aug 14, 1975·Nature·D S Gilbert, B J Newby
Sep 1, 1978·Analytical Biochemistry·D C RuegerA Bignami
Oct 29, 1976·Brain Research·D Dahl, A Bignami
Sep 1, 1977·Journal of Neurochemistry·K IqbalR D Terry
Jul 15, 1976·FEBS Letters·W E BenitzA Bignami
Dec 24, 1976·Brain Research·W J Schook, W T Norton
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M L ShelanskiC R Cantor
Mar 24, 1972·Science·G H DeVriesC S Raine
Jan 1, 1974·Journal of Neurobiology·P F Davison, B Winslow
May 7, 1971·Brain Research·L F EngB Gerstl
Dec 17, 1971·Science·M L ShelanskiW T Norton

Related Concepts

Neurofilament
Glial Fibrillary Acidic Protein
Astrocytes
Immune Sera
Bos taurus
Bromides Measurement
Immunoaffinity Chromatography
Urea Measurement
Brain Chemistry
Polypeptides

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