Asymmetric distribution of the Listeria monocytogenes ActA protein is required and sufficient to direct actin-based motility

Molecular Microbiology
G A SmithJ A Theriot

Abstract

Listeria monocytogenes is a Gram-positive facultative intracytoplasmic bacterial pathogen that exhibits rapid actin-based motility in eukaryotic cells and in cell-free cytoplasmic extracts. The protein product of the actA gene is required for bacterial movement and is normally expressed in a polarized fashion on the bacterial surface. Here we demonstrate that the ActA protein is sufficient to direct motility in the absence of other L. monocytogenes gene products, and that polarized localization of the protein is required for efficient unidirectional movement. We have engineered a fusion protein combining ActA with the C-terminal domain of the LytA protein of Streptococcus pneumoniae, which mediates high-affinity binding to DEAE-cellulose and to choline moieties present in the S. pneumoniae cell wall. DEAE-cellulose fragments or S. pneumoniae coated uniformly with the ActA/LytA fusion protein nucleate actin filament growth in cytoplasmic extracts, but do not move efficiently. However, when ActA/LytA-coated S. pneumoniae is grown to polarize the distribution of the fusion protein, the bacteria exhibit unidirectional actin-based movement similar to the normal movement of L. monocytogenes.

Citations

Dec 1, 1996·Biophysical Journal·A Mogilner, G Oster
Feb 5, 2000·Biochimica Et Biophysica Acta·D J Olbris, J Herzfeld
Feb 1, 1996·Current Opinion in Cell Biology·S H Zigmond
Feb 1, 1997·Current Opinion in Cell Biology·S Higley, M Way
Jul 22, 1998·Nature·L M Machesky, M Way
Jan 18, 2006·Nature Reviews. Microbiology·Joanne M StevensMark P Stevens
Jan 21, 2012·The Journal of Biological Chemistry·Shivani AgarwalVijay Pancholi
Jun 26, 2008·The Journal of Biological Chemistry·Matthew J FooterJulie A Theriot
Jul 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·R LópezJ L García
Mar 9, 2004·Molecular Biology of the Cell·Lisa A CameronJulie A Theriot
Oct 1, 2004·Molecular Biology of the Cell·Patricia T Yam, Julie A Theriot
Jan 6, 2012·Molecular Biology of the Cell·Catherine I LacayoJulie A Theriot
Mar 31, 2012·Molecular Biology of the Cell·Daniel A Portnoy
May 11, 2010·Cold Spring Harbor Perspectives in Biology·David Z Rudner, Richard Losick
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
May 10, 2001·Journal of Bacteriology·S R Lybarger, J R Maddock
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·M B Goldberg
Jan 19, 1999·Annual Review of Cell and Developmental Biology·S Dramsi, P Cossart
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Matthew D Welch, R Dyche Mullins
Jun 7, 2006·Annual Review of Microbiology·June R Scott, Timothy C Barnett
Sep 23, 2009·PLoS Biology·Mark J DayelR Dyche Mullins
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·P MourrainP Cossart
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·L A CameronJ A Theriot
Nov 12, 2014·Biochemistry. Biokhimii︠a︡·S Yu Khaitlina
Oct 1, 1996·Molecular Microbiology·R C SandlinA T Maurelli
Sep 24, 1999·The Journal of Cell Biology·J R RobbinsJ A Theriot
Oct 21, 2004·The Journal of Pathology·Klemens RottnerTheresia E B Stradal
Aug 28, 2007·Microbes and Infection·Pamela Schnupf, Daniel A Portnoy
Jan 25, 2006·Molecular Microbiology·Susanne M Rafelski, Julie A Theriot

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.