Asymmetrically dividing Drosophila neuroblasts utilize two spatially and temporally independent cytokinesis pathways

Nature Communications
Michaela RothClemens Cabernard

Abstract

Precise cleavage furrow positioning is required for faithful chromosome segregation and cell fate determinant distribution. In most metazoan cells, contractile ring placement is regulated by the mitotic spindle through the centralspindlin complex, and potentially also the chromosomal passenger complex (CPC). Drosophila neuroblasts, asymmetrically dividing neural stem cells, but also other cells utilize both spindle-dependent and spindle-independent cleavage furrow positioning pathways. However, the relative contribution of each pathway towards cytokinesis is currently unclear. Here we report that in Drosophila neuroblasts, the mitotic spindle, but not polarity cues, controls the localization of the CPC component Survivin. We also show that Survivin and the mitotic spindle are required to stabilize the position of the cleavage furrow in late anaphase and to complete furrow constriction. These results support the model that two spatially and temporally separate pathways control different key aspects during asymmetric cell division, ensuring correct cell fate determinant segregation and neuroblast self-renewal.

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Citations

Mar 12, 2016·Seminars in Cell & Developmental Biology·Masanori Mishima
Sep 30, 2015·The Journal of Cell Biology·Anne PacqueletGrégoire Michaux
Aug 10, 2020·Current Opinion in Cell Biology·Melissa K Delgado, Clemens Cabernard

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Methods Mentioned

BETA
GTPase
fluorescence recovery after photobleaching
super-resolution microscopy
dissections
PCR
irradiate

Software Mentioned

DeltaVision OMX SoftWoRx
Matlab
Imaris
Fiji
Corel Draw Photo
Photoshop
Graphpad Prism
Illustrator
ImageJ
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