PMID: 11934444Apr 6, 2002Paper

Asymmetry of frameshift mutagenesis during leading and lagging-strand replication in Escherichia coli

Mutation Research
Damian GawelIwona J Fijalkowska

Abstract

Mutations in DNA, including frameshifts, may arise during DNA replication as a result of mistakes made by the DNA polymerase in copying the DNA template strands. In our efforts to better understand the factors that contribute to the accuracy of DNA replication, we have investigated whether frameshift mutations on the Escherichia coli chromosome occur differentially within the leading and lagging-strands of replication. The experimental system involves measurement of the reversion frequency for several defined lac frameshift alleles in pairs of strains in which the lac target is oriented in the two possible directions relative to the origin of chromosomal replication. Within these pairs any defined lac sequence will be subject to leading-strand replication in one orientation and to lagging-strand replication in the other. Fidelity differences between the two modes of replication can be observed as a differential lac reversion between the two strains. Our results, obtained with a series of lac alleles in a mismatch-repair-defective background, indicate that for at least some of the alleles there is indeed a difference in the fidelity of replication between the two modes of replication.

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Citations

May 7, 2003·Current Opinion in Microbiology·Jean R Lobry, Jean-Michel Louarn
Oct 28, 2006·Genome Research·Eduardo P C RochaEdward J Feil
Sep 19, 2006·Journal of Bacteriology·Wojciech KubanIwona J Fijalkowska
Jul 27, 2002·Journal of Bacteriology·Damian GawelIwona J Fijalkowska
Jul 3, 2004·Journal of Bacteriology·Wojciech KubanIwona J Fijalkowska
Apr 12, 2007·Genome Génome / Conseil National De Recherches Canada·Aïda AzaiezFrançois J Belzile
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Aug 10, 2013·FEMS Microbiology Reviews·Kai ZhouChris W Michiels
Mar 13, 2012·FEMS Microbiology Reviews·Iwona J FijalkowskaPiotr Jonczyk
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