AT-rich sequences from the arbuscular mycorrhizal fungus Gigaspora rosea exhibit ARS function in the yeast Saccharomyces cerevisiae

Fungal Genetics and Biology : FG & B
Roberta Bergero

Abstract

Autonomous replicating sequences are DNA elements that trigger DNA replication and are widely used in the development of episomal transformation vectors for fungi. In this paper, a genomic library from the mycorrhizal fungus Gigaspora rosea was constructed in the integrative plasmid YIp5 and screened in the budding yeast Saccharomyces cerevisiae for sequences that act as ARS and trigger plasmid replication. Two genetic elements (GrARS2, GrARS6) promoted high-rates of yeast transformation. Sequence analysis of these elements shows them to be AT-rich (72-80%) and to contain multiple near-matches to the yeast autonomous consensus sequences ACS and EACS. GrARS2 contained a putative miniature inverted-repeat transposable element (MITE) delimited by 28-bp terminal inverted repeats (TIRs). Disruption of this element and removal of one TIR increased plasmid stability several fold. The potential for palindromes to affect DNA replication is discussed.

References

Jul 25, 1992·Nucleic Acids Research·K Robzyk, Y Kassir
Aug 1, 1990·Molecular and Cellular Biology·J V Van Houten, C S Newlon
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J F Diffley, B Stillman
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J L Revuelta, M Jayaram
Dec 25, 1984·Journal of Molecular Biology·D T Weaver, M L DePamphilis
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C S Chan, B K Tye
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Rao, B Stillman
Nov 1, 1994·Molecular and Cellular Biology·H RaoB Stillman
Sep 1, 1993·Molecular and Cellular Biology·D A GordeninM A Resnick
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J F Theis, C S Newlon
Feb 20, 2004·Nature·Teresa E Pawlowska, John W Taylor
Dec 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianli DaiThomas J Kelly
Jul 19, 2005·Molecular and Cellular Biology·Ronald Lebofsky, Aaron Bensimon

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.