AT1a influences GABAA-mediated inhibition through regulation of KCC2 expression

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
George E FarmerJ Thomas Cunningham

Abstract

The median preoptic nucleus (MnPO) is an integrative site involved in body fluid homeostasis, cardiovascular control, thermoregulation, and sleep homeostasis. Angiotensin II (ANG II), a neuropeptide shown to have excitatory effects on MnPO neurons, is of particular interest with regard to its role in body fluid homeostasis and cardiovascular control. The present study investigated the role of angiotensin type 1a (AT1a) receptor activation on neuronal excitability in the MnPO. Male Sprague-Dawley rats were infused with an adeno-associated virus with an shRNA against the AT1a receptor or a scrambled control. In vitro loose-patch voltage-clamp recordings of spontaneous action potential activity were made from labeled MnPO neurons in response to brief focal application of ANG II or the GABAA receptor agonist muscimol. Additionally, tissue punches from MnPO were taken to asses mRNA and protein expression. AT1a receptor knockdown neurons were insensitive to ANG II and showed a marked reduction in GABAA-mediated inhibition. The reduction in GABAA-mediated inhibition was not associated with reductions in mRNA or protein expression of GABAA β-subunits. Knockdown of the AT1a receptor was associated with a reduction in the potassium-chlor...Continue Reading

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Citations

Jun 21, 2019·Scientific Reports·George E FarmerJ Thomas Cunningham
Jul 23, 2020·Journal of Neurophysiology·Alexandria B MarcianteJ Thomas Cunningham
May 29, 2019·Journal of Neuroendocrinology·Kirthikaa BalapattabiJ Thomas Cunningham
Feb 18, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alexandria B MarcianteJ Thomas Cunningham
Jul 1, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·George E FarmerJ Thomas Cunningham

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Datasets Mentioned

BETA
AB5561

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ImageJ

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