AtDRO1 is nuclear localized in root tips under native conditions and impacts auxin localization

Plant Molecular Biology
Jessica M WaiteChris Dardick

Abstract

DEEPER ROOTING 1 (DRO1) contributes to the downward gravitropic growth trajectory of roots upstream of lateral auxin transport in monocots and dicots. Loss of DRO1 function leads to horizontally oriented lateral roots and altered gravitropic set point angle, while loss of all three DRO family members results in upward, vertical root growth. Here, we attempt to dissect the roles of AtDRO1 by analyzing expression, protein localization, auxin gradient formation, and auxin responsiveness in the atdro1 mutant. Current evidence suggests AtDRO1 is predominantly a membrane-localized protein. Here we show that VENUS-tagged AtDRO1 driven by the native AtDRO1 promoter complemented an atdro1 Arabidopsis mutant and the protein was localized in root tips and detectable in nuclei. atdro1 primary and lateral roots showed impairment in establishing an auxin gradient upon gravistimulation as visualized with DII-VENUS, a sensor for auxin signaling and proxy for relative auxin distribution. Additionally, PIN3 domain localization was not significantly altered upon gravistimulation in atdro1 primary and lateral roots. RNA-sequencing revealed differential expression of known root development-related genes in atdro1 mutants. atdro1 lateral roots were ...Continue Reading

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Citations

Jan 11, 2021·Current Opinion in Plant Biology·Jessica Marie Waite, Christopher Dardick
Apr 27, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Eric S OberMichelle Watt
May 1, 2021·Plants·Shiwangni RaoGarry M Rosewarne
Jun 3, 2021·International Journal of Molecular Sciences·Manvi SharmaAshverya Laxmi

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Methods Mentioned

BETA
confocal microscopy
RNA-seq
transgenic
genotyping
RNA Extraction

Software Mentioned

VENUS
TOPLESS
ImageJ
CLC Genomics Workbench
Image J
BiFC

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