ATF site of human RB gene promoter is a responsive element of myogenic differentiation

FEBS Letters
Y OkuyamaT Sakai

Abstract

RB mRNA increases during terminal differentiation of C2 myoblasts. We demonstrate that RB promoter activity increases about 4-fold during differentiation. The increase of RB promoter activity was reduced when a point mutation was designed in the ATF site. In a gel shift assay of the ATF site, two specific bands were observed. One of them, with the lower mobility, disappeared during differentiation. This band reacted with an antibody against ATF-1. We cotransfected an RB promoter-luciferase plasmid with the TREB36/ATF-1 plasmid. ATF-l suppressed the activity of the wild-type RB promoter but not of that with a point mutation at the ATF site. These results suggest that the ATF site of the RB promoter is a responsive element during myogenic differentiation of C2 cells. We hypothesize that RB promoter activity is stimulated partially due to the dissociation of ATF-1, which suppresses the promoter activity through the ATF site in C2 myoblasts.

References

Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jan 1, 1994·Molecular and Cellular Biology·B ShanW H Lee

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Citations

Jun 15, 2007·The Journal of Biological Chemistry·Kenta IwasakiYoshiaki Tsuji
Jan 31, 2004·Nucleic Acids Research·Masayuki Sekimata, Yoshimi Homma
Jun 2, 2006·Molecular and Cellular Biology·Monica AgromayorLili Yamasaki
Apr 1, 2003·Molecular and Cellular Biology·Alessandra MagentaArmando Felsani
Oct 29, 2010·Journal of Cell Science·Kiros HailemariamYoshiaki Tsuji
Dec 25, 2004·The International Journal of Biochemistry & Cell Biology·Mark D Temple, Vincent Murray
May 29, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Guo-Liang HuangZhi-Wei He
May 25, 2002·Experimental Cell Research·Yan DongBonnie B Asch
Dec 21, 2000·The Journal of Cell Biology·A PorrelloS Soddu
Jun 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shang-Chih YangJean Lu

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