Atg7-Mediated Autophagy Is Involved in the Neural Crest Cell Generation in Chick Embryo

Molecular Neurobiology
Guang WangXuesong Yang

Abstract

Autophagy plays a very important role in numerous physiological and pathological events. However, it still remains unclear whether Atg7-induced autophagy is involved in the regulation of neural crest cell production. In this study, we found the co-location of Atg7 and Pax7+ neural crest cells in early chick embryo development. Upregulation of Atg7 with unilateral transfection of full-length Atg7 increased Pax7+ and HNK-1+ cephalic and trunk neural crest cell numbers compared to either Control-GFP transfection or opposite neural tubes, suggesting that Atg7 over-expression in neural tubes could enhance the production of neural crest cells. BMP4 in situ hybridization and p-Smad1/5/8 immunofluorescent staining demonstrated that upregulation of Atg7 in neural tubes suppressed the BMP4/Smad signaling, which is considered to promote the delamination of neural crest cells. Interestingly, upregulation of Atg7 in neural tubes could significantly accelerate cell progression into the S phase, implying that Atg7 modulates cell cycle progression. However, β-catenin expression was not significantly altered. Finally, we demonstrated that upregulation of the Atg7 gene could activate autophagy as did Atg8. We have also observed that similar phen...Continue Reading

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Citations

Nov 2, 2018·Histochemistry and Cell Biology·Eric Bekoe OffeiBeate Brand-Saberi
Dec 7, 2018·Zygote : the Biology of Gametes and Early Embryos·Xiao-Tan ZhangXuesong Yang

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Datasets Mentioned

BETA
BC000091

Methods Mentioned

BETA
transfecting
transfection
delamination

Software Mentioned

Pro Plus
Olympus
Image
SPSS
Quantity One

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