Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Hirosumi TamuraYasuo Uchiyama

Abstract

Old and unneeded intracellular macromolecules are delivered through autophagy to lysosomes that degrade macromolecules into bioactive monomers such as amino acids. Autophagy is conserved in eukaryotes and is essential for the maintenance of cellular metabolism. Currently, more than 30 autophagy-related genes (Atgs) have been identified in yeast. Of these genes, the18 that are essential for autophagosome formation are also conserved in mammalian cells. Atg9 is the only transmembrane Atg protein required for autophagosome formation. Although the subcellular localization of the Atg9A protein (Atg9Ap) has been examined, little is known about its precise cell and tissue distribution. To determine this, we produced an antibody specific to mouse Atg9Ap. The antibody recognized both non-glycosylated and glycosylated Atg9Ap, which have molecular masses of approximately 94 kDa and 105 kDa, respectively. Although Atg9Ap was ubiquitously detected, it was highly expressed in neurons of the central nervous system. In Purkinje cells, Atg9Ap immunoreactivity was localized in the endoplasmic reticulum (ER), trans-Golgi network (TGN), lysosomes/late endosomes, and in axon terminals. These results suggest that Atg9Ap may be involved in autophagos...Continue Reading

References

Jan 1, 1987·Annual Review of Nutrition·G E Mortimore, A R Pösö
Mar 29, 2000·Journal of Bacteriology·T LangM Thumm
Sep 21, 2000·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·M KoikeY Uchiyama
Dec 2, 2000·Science·D J Klionsky, S D Emr
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·Y Ohsumi
Oct 11, 2003·Developmental Cell·Daniel J KlionskyYoshinori Ohsumi
Dec 20, 2003·Biochemical and Biophysical Research Communications·Alfred J Meijer, Peter F Dubbelhuis
Nov 5, 2004·Nature·Akiko KumaNoboru Mizushima
Jul 29, 2006·Autophagy·Fulvio ReggioriDaniel J Klionsky
Aug 31, 2006·Journal of Cell Science·Andrew R J YoungSharon A Tooze
Nov 15, 2006·Autophagy·Jemma L WebberSharon A Tooze
Feb 14, 2007·Autophagy·Wei-Lien Yen, Daniel J Klionsky
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Masaaki KomatsuZhenyu Yue
Nov 17, 2007·Genes & Development·Noboru Mizushima
Mar 6, 2008·Histochemistry and Cell Biology·Yasuo UchiyamaMitsuho Sasaki
Aug 15, 2008·Molecular Biology of the Cell·R KrickM Thumm
Apr 18, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takayuki SekitoYoshinori Ohsumi
May 23, 2009·Journal of Cell Science·Brinda RavikumarDavid C Rubinsztein
Oct 27, 2009·Autophagy·Päivi Ylä-AnttilaEeva-Liisa Eskelinen
Nov 10, 2009·Nature Cell Biology·Mitsuko Hayashi-NishinoAkitsugu Yamamoto
Nov 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tatsuya SaitohShizuo Akira
Oct 12, 2010·Autophagy·Nicholas T KtistakisJoshua Long

❮ Previous
Next ❯

Citations

Apr 17, 2015·Biomedical Research·Tomohisa NanaoYasuo Uchiyama
Aug 6, 2013·Neurobiology of Aging·Sara SepeSandra Moreno
Sep 12, 2012·Autophagy·Daniel J KlionskyBrian Zuckerbraun
Jun 19, 2016·Biochimica Et Biophysica Acta·Catherine StaudtMichel Jadot
Jul 1, 2017·Cells·Wim MartinetGuido R Y De Meyer
Mar 24, 2017·The EMBO Journal·Roeland VanhauwaertPatrik Verstreken
Jun 19, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yoshiyuki ArataShigeo Murata
Jul 13, 2012·The Journal of Biological Chemistry·Mariana PeharLuigi Puglielli
Oct 17, 2019·Frontiers in Cellular Neuroscience·Dominik R GabrychMichael A Silverman
Jan 21, 2016·Brain : a Journal of Neurology·Yajing PengLuigi Puglielli
Dec 29, 2020·Cell Reports·José WojnackiThierry Galli
Mar 20, 2020·Neuron·Sarah E Hill, Daniel A Colón-Ramos

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
PCR
confocal microscopy
glycosylation
electron

Software Mentioned

Image Gauge

Related Concepts

Related Feeds

Autophagy Networks

Autophagy is a lysosomal pathway that involves degradation of proteins and functions in normal growth and pathological conditions, through a series of complex networks. The catabolic process involves delivery of proteins and organelles to the lysosome. Here is the latest research on autophagy networks.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Cell Death and Differentiation
A Longatti, Sharon A Tooze
Seminars in Cell & Developmental Biology
Sharon A Tooze
FEBS Letters
Jemma L Webber, Sharon A Tooze
Cell Death and Differentiation
T Yorimitsu, D J Klionsky
© 2021 Meta ULC. All rights reserved