ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6.

Journal of Lipid Research
Thomas O EichmannAchim Lass

Abstract

Lipid droplets (LDs) of hepatic stellate cells (HSCs) contain large amounts of vitamin A [in the form of retinyl esters (REs)] as well as other neutral lipids such as TGs. During times of insufficient vitamin A availability, RE stores are mobilized to ensure a constant supply to the body. To date, little is known about the enzymes responsible for the hydrolysis of neutral lipid esters, in particular of REs, in HSCs. In this study, we aimed to identify LD-associated neutral lipid hydrolases by a proteomic approach using the rat stellate cell line HSC-T6. First, we loaded cells with retinol and FAs to promote lipid synthesis and deposition within LDs. Then, LDs were isolated and lipid composition and the LD proteome were analyzed. Among other proteins, we found perilipin 2, adipose TG lipase (ATGL), and comparative gene identification-58 (CGI-58), known and established LD proteins. Bioinformatic search of the LD proteome for α/β-hydrolase fold-containing proteins revealed no yet uncharacterized neutral lipid hydrolases. In in vitro activity assays, we show that rat (r)ATGL, coactivated by rat (r)CGI-58, efficiently hydrolyzes TGs and REs. These findings suggest that rATGL and rCGI-58 are LD-resident proteins in HSCs and participa...Continue Reading

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Citations

Jan 26, 2016·Nitric Oxide : Biology and Chemistry·Alexander KollauBernd Mayer
Dec 8, 2015·Experimental Cell Research·Wen-Jun ShenFredric B Kraemer
May 15, 2016·Journal of Lipid Research·Maidina TuohetahuntilaJ Bernd Helms
May 25, 2018·Journal of Cell Science·Francesca NardiSusan E Crawford
Sep 15, 2018·Proteomics·Congyan Zhang, Pingsheng Liu
Jun 30, 2016·The Journal of Biological Chemistry·Lukas GrumetAchim Lass
Nov 25, 2020·Cell Metabolism·Parth TrivediScott L Friedman
May 17, 2017·Advanced Drug Delivery Reviews·Takaaki HigashiYujin Hoshida

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Methods Mentioned

BETA
light scattering
PCR

Software Mentioned

Empower
CDSearch Specific BLAST ( RPSBLAST )
SpectrumMill
Lipid Data Analyzer
MassLynx
MASPECTRAS
ChemStation

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