Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion

Biochemical and Biophysical Research Communications
Dongjin ShinDae-Jin Yun

Abstract

An Arabidopsis cDNA clone that encodes Athb-12, a homeobox-leucine zipper domain protein (HD-Zip), was isolated by functional complementation of the NaCl-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant (cnbDelta, regulatory subunit null). CaN, a Ca2+/calmodulin-dependent protein phosphatase, regulates Na+ ion homeostasis in yeast. Expression of Athb-12 increased NaCl tolerance but not osmotic stress tolerance of these cnbDelta cells. Furthermore, expression of two other HD-Zip from Arabidopsis, Athb-1 and -7, did not suppress NaCl sensitivity of cnbDelta cells. These results suggest that Athb-12 specifically functions in Na+ ion homeostasis in yeast. Consistent with these observations, expression of Athb-12 in yeast turned on transcription of the NaCl stress-inducible PMR2A, which encodes a Na+/Li+ translocating P-type ATPase, and decreased Na+ levels in yeast cells. To investigate the biological function of Athb-12 in Arabidopsis, we performed Northern blot analysis. Expression of Athb-12 was dramatically induced by NaCl and ABA treatments, but not by KCl. In vivo targeting experiments using a green fluorescent protein reporter indicated that Athb-12 was localized to the nucleus. These results suggest that At...Continue Reading

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Citations

Dec 1, 2006·Functional & Integrative Genomics·Grant R CramerJohn C Cushman
Apr 18, 2007·Plant Molecular Biology·Antonio F MonroyPatrick J Gulick
Aug 8, 2014·International Journal of Molecular Sciences·Senlei ZhangXia Li
Mar 21, 2006·Journal of Plant Physiology·Yucheng WangChuanping Yang
Oct 28, 2008·Annals of Botany·Dong DingYonglian Zheng
May 26, 2021·Plant Physiology and Biochemistry : PPB·Marcos Fernando BassoMaria Fatima Grossi-de-Sa

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