AtLa1 protein initiates IRES-dependent translation of WUSCHEL mRNA and regulates the stem cell homeostasis of Arabidopsis in response to environmental hazards

Plant, Cell & Environment
Yuchao CuiTao Huang

Abstract

Plant stem cells are hypersensitive to environmental hazards throughout their life cycle, but the mechanism by which plants safeguard stem cell homeostasis in response to environmental hazards is largely unknown. The homeodomain transcription factor WUSCHEL (WUS) protein maintains the stem cell pool in the shoot apical meristem of Arabidopsis. Here, we demonstrate that the translation of WUS mRNA is directed by an internal ribosomal entry site (IRES) located in the 5'-untranslated region. The AtLa1 protein, an RNA-binding factor, binds to the 5'-untranslated region and initiates the IRES-dependent translation of WUS mRNA. Knockdown of AtLa1 expression represses the WUS IRES-dependent translation and leads to the arrest of growth and development. The AtLa1 protein is mainly located in the nucleoplasm. However, environmental hazards promote the nuclear-to-cytoplasmic translocation of the AtLa1 protein, which further enhances the IRES-dependent translation of WUS mRNA. Genetic evidence indicates that the WUS protein increases the tolerance of the shoot apical meristem to environmental hazards. Based on these results, we conclude that the stem cell niche in Arabidopsis copes with environmental hazards by enhancing the IRES-dependen...Continue Reading

References

Feb 1, 1989·The Journal of Cell Biology·M Kozak
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·L B BlynE Ehrenfeld
Aug 1, 1997·Journal of Virology·L B BlynE Ehrenfeld
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
May 16, 2001·The Journal of Cell Biology·R J Maraia
Jul 11, 2001·Genes & Development·C U Hellen, P Sarnow
Jan 29, 2002·Nucleic Acids Research·Y K KimS K Jang
Jun 5, 2002·Annual Review of Biochemistry·Sandra L Wolin, Tommy Cedervall
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hannah Miriam JaagDirk Prüfer
Jul 4, 2003·The Journal of Biological Chemistry·Beatrice CardinaliPaola Pierandrei-Amaldi
Dec 6, 2003·The EMBO Journal·Ghadiyaram ChakshusmathiSandra L Wolin
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Maki Inada, Christine Guthrie
Feb 11, 2005·The Plant Journal : for Cell and Molecular Biology·Tzvetanka D DinkovaEstela Sánchez de Jiménez
Oct 8, 2005·Science·G Venugopala Reddy, Elliot M Meyerowitz
Nov 30, 2005·The New Phytologist·Clifford M Bray, Christopher E West
Apr 21, 2006·Molecular Biology of the Cell·Sarah CollierPeter Shaw
Apr 27, 2007·Nucleic Acids Research·Sophie FleurdépineCécile Bousquet-Antonelli
May 8, 2009·The Plant Journal : for Cell and Molecular Biology·Lionel C Terzi, Gordon G Simpson
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nick Fulcher, Robert Sablowski
Feb 5, 2010·The Plant Journal : for Cell and Molecular Biology·Sascha Biedermann, Hanjo Hellmann

❮ Previous
Next ❯

Citations

Dec 20, 2015·Trends in Plant Science·Roger P HellensRichard C Macknight
Mar 15, 2016·Journal of Plant Physiology·Katarzyna Nowak, Małgorzata D Gaj
Mar 1, 2017·The Plant Journal : for Cell and Molecular Biology·Catharina MerchanteJose M Alonso
Jul 15, 2017·Plant, Cell & Environment·Vanesa B TognettiMónika Hrtyan
Aug 2, 2017·Proteomics·Polly Yingshan Hsu, Philip N Benfey
Apr 26, 2019·Journal of Experimental Botany·Xiaolong ChenRongyu Huang
Jan 29, 2020·Journal of Experimental Botany·Min-Liang WuHong Cui
Jul 7, 2017·Journal of Integrative Plant Biology·Xuehuan DaiFengning Xiang
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.