Oct 26, 2018

Atlas of Subcellular RNA Localization Revealed by APEX-seq

BioRxiv : the Preprint Server for Biology
Furqan M FazalAlice Y Ting

Abstract

We introduce APEX-seq, a method for RNA sequencing based on spatial proximity to the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales produced a nanometer-resolution spatial map of the human transcriptome, revealing extensive and exquisite patterns of localization for diverse RNA classes and transcript isoforms. We uncover a radial organization of the nuclear transcriptome, which is gated at the inner surface of the nuclear pore for cytoplasmic export of processed transcripts. We identify two distinct pathways of messenger RNA localization to mitochondria, each associated with specific sets of transcripts for building complementary macromolecular machines within the organelle. APEX-seq should be widely applicable to many systems, enabling comprehensive investigations of the spatial transcriptome.

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Mentioned in this Paper

Establishment and Maintenance of Localization
Biochemical Pathway
Apex Cardiography
Sequence Determinations, RNA
Peroxidases
Mitochondria
Cytoplasmic
Macromolecule
APEX1
Cellular Component Organization

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