Atlas of tissue- and developmental stage specific gene expression for the bovine insulin-like growth factor (IGF) system

PloS One
Mani Ghanipoor-SamamiStefan Hiendleder

Abstract

The insulin-like growth factor (IGF) axis is fundamental for mammalian growth and development. However, no comprehensive reference data on gene expression across tissues and pre- and postnatal developmental stages are available for any given species. Here we provide systematic promoter- and splice variant specific information on expression of IGF system components in embryonic (Day 48), fetal (Day 153), term (Day 277, placenta) and juvenile (Day 365-396) tissues of domestic cow, a major agricultural species and biomedical model. Analysis of spatiotemporal changes in expression of IGF1, IGF2, IGF1R, IGF2R, IGFBP1-8 and IR genes, as well as lncRNAs H19 and AIRN, by qPCR, indicated an overall increase in expression from embryo to fetal stage, and decrease in expression from fetal to juvenile stage. The stronger decrease in expression of lncRNAs (average -16-fold) and ligands (average -12.1-fold) compared to receptors (average -5.7-fold) and binding proteins (average -4.3-fold) is consistent with known functions of IGF peptides and supports important roles of lncRNAs in prenatal development. Pronounced overall reduction in postnatal expression of IGF system components in lung (-12.9-fold) and kidney (-13.2-fold) are signatures of m...Continue Reading

References

Mar 1, 1977·British Journal of Obstetrics and Gynaecology·L C van OtterloH C Wallenburg
Jul 11, 1992·Biological Signals·D LeRoithC T Roberts
Sep 30, 1992·Biochemical and Biophysical Research Communications·E JansenJ S Sussenbach
Apr 1, 1991·The Journal of Clinical Endocrinology and Metabolism·F LiuR L Hintz
May 9, 1991·Nature·M S BartolomeiS M Tilghman
Oct 1, 1991·Molecular and Cellular Endocrinology·M A van DijkJ S Sussenbach
May 11, 1991·Molecular and Cellular Endocrinology·D LeRoith, C T Roberts
Jun 1, 1991·Molecular and Cellular Endocrinology·J V O'MahoneyT E Adams
Feb 22, 1991·Cell·T M DeChiaraA Efstratiadis
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 30, 1990·Biochimica Et Biophysica Acta·P HolthuizenJ S Sussenbach
Nov 1, 1989·Endocrinology·S M DonovanR G Rosenfeld
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WernerD LeRoith
Aug 1, 1989·Molecular Endocrinology·D E MollerJ S Flier
Apr 20, 1987·FEBS Letters·P de Pagter-HolthuizenJ S Sussenbach
Dec 1, 1987·Endocrinology·P RotweinJ D Milbrandt
Jul 1, 1985·The American Journal of Physiology·S TomodaL D Longo
Oct 1, 1984·The Journal of Clinical Endocrinology and Metabolism·T J MerimeeJ E Tyson
Apr 1, 1995·Endocrine Reviews·D LeRoithC T Roberts
May 1, 1993·Molecular and Cellular Endocrinology·C Y LeeF A Simmen
Oct 1, 1993·Proceedings of the Society for Experimental Biology and Medicine·P D LeeD R Powell
Jul 1, 1994·Molecular Reproduction and Development·O LustigA Hochberg
May 1, 1993·Nature Genetics·N GiannoukakisC Polychronakos
Oct 20, 1993·Regulatory Peptides·P E HolthuizenJ S Sussenbach
Dec 15, 1993·Biochemical and Biophysical Research Communications·Y XuC Polychronakos
Aug 1, 1993·Molecular Reproduction and Development·P NissleyM Sklar

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Methods Mentioned

BETA
PCR
cesarean section
electrophoresis
dissection

Software Mentioned

Quantity One
Mastercycler ® ep Realplex
SPSS
NormFinder
Basic Local Alignment Search Tool
Ensembl

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