Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices

Rapid Communications in Mass Spectrometry : RCM
Kornél NagyKároly Vékey

Abstract

Application of high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) for detection of aldehydes in biological samples such as blood, yoghurt, and milk, is reported. Sample preparation is easy, and the presented method is both sensitive and selective. It is based on the widely used dinitrophenylhydrazine derivatization, followed by extraction with n-hexane and a simple reversed-phase HPLC separation. Detection is performed by atmospheric pressure chemical ionization (APCI) in negative ion mode, with detection limits in the low picogram range. Using MS/MS, acetone and propionaldehyde can clearly be distinguished, facilitating propionaldehyde quantitation even in the presence of high acetone levels. Quantitation by direct MS/MS is also feasible, well suited for high-throughput applications, although not as accurate as using HPLC/MS/MS.

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Citations

Dec 24, 2011·Bioanalysis·Pan DengDafang Zhong
Mar 20, 2010·Analytical and Bioanalytical Chemistry·Mark EgginkHubertus Irth
Sep 21, 2011·Biochimica Et Biophysica Acta·Evgeny V Berdyshev
Jul 4, 2007·Journal of Environmental Monitoring : JEM·Raymond OlsenPaal Molander
Feb 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Clara Eugenia Baños, Manuel Silva
Nov 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mark EgginkHubertus Irth
Sep 8, 2011·Alcoholism, Clinical and Experimental Research·Xiangying GuanHelen Anni
Jun 17, 2014·Mass Spectrometry Reviews·D Allan ButterfieldRenã A S Robinson
Jul 4, 2006·Journal of Chromatography. a·J Ruiz-Jiménez, M D Luque de Castro
May 26, 2006·European Journal of Mass Spectrometry·Vladimir G Zaikin, John M Halket
Aug 17, 2017·Nature·Guillermo Burgos-BarraganKetan J Patel
Oct 22, 2020·Biomedical Chromatography : BMC·Victor DavidToma Galaon

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