PMID: 3768360Nov 6, 1986Paper

Atomic and Raman spectroscopy of the dipalmitoylphosphatidic acid-calcium complex and phase transitions

Biochimica Et Biophysica Acta
E Bicknell-BrownD Borchman

Abstract

Calcium binding measurements by atomic absorption spectroscopy and temperature-dependent phase transitions studies by Raman spectroscopy were combined in order to investigate the effect of Ca2+ binding on dipalmitoylphosphatidic acid (DPPA) dispersed in CaCl2 solutions of varying concentration at pH 7. The peak heights for the Raman CH stretch bands observed at 2885 cm-1 and 2935 cm-1 were used as a measure of hydrocarbon chain randomization and aggregate ultrastructure. Two transitions were observed for both pure DPPA and DPPA-Ca2+ mixtures. Ca2+ binding caused greatly increased DPPA chain rigidity in the melted state above Tm, but had much less effect on the solid phases below Tm. The increase in rigidity in the fluid state was observed to vary linearly with the molar ratio of bound Ca2+ to total DPPA throughout the range 0 to 1. The results of the Raman and Ca2+ binding measurements are explained by a model in which two populations of DPPA co-exist in the fluid state when Ca2+ binding has not reached saturation. One population consists of the Ca2+-bound DPPA complex with stoichiometric 1:1 binding ratio (determined from an atomic absorption Ca2+ binding study), and the second population is free DPPA. We propose that Ca2+-ind...Continue Reading

References

Feb 16, 1976·European Journal of Biochemistry·H HauserM C Phillips
Jul 3, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosG Poste
Jul 3, 1975·Biochimica Et Biophysica Acta·J Lansman, D H Haynes
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Träuble, H Eibl
Sep 5, 1973·Biochemical and Biophysical Research Communications·K G BrownE Brown
May 30, 1974·Biochimica Et Biophysica Acta·D PapahadjopoulosW J Vail
Jul 25, 1984·Biochimica Et Biophysica Acta·V W Miner, J H Prestegard
Mar 1, 1977·Biochimica Et Biophysica Acta·B P Gaber, W L Peticolas

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Citations

May 23, 2002·Biophysical Journal·Madalina RujoiM Cecilia Yappert
Nov 17, 2009·Biochimica Et Biophysica Acta·Anders BjörkbomJ Peter Slotte
Feb 27, 2009·The Journal of Physical Chemistry. B·Emily R LambersonJennifer S Hovis
May 9, 1990·Biochimica Et Biophysica Acta·J Sun, M Petersheim

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