PMID: 9427660Mar 7, 1998Paper

Atomic force microscopic imaging of seeded fibril formation and fibril branching by the Alzheimer's disease amyloid-beta protein

Chemistry & Biology
J D HarperP T Lansbury

Abstract

Amyloid plaques composed of the fibrillar form of the amyloid-beta protein (Abeta) are the defining neuropathological feature of Alzheimer's disease (AD). A detailed understanding of the time course of amyloid formation could define steps in disease progression and provide targets for therapeutic intervention. Amyloid fibrils, indistinguishable from those derived from an AD brain, can be produced in vitro using a seeded polymerization mechanism. In its simplest form, this mechanism involves a cooperative transition from monomeric Abeta to the amyloid fibril without the buildup of intermediates. Recently, however, a transient species, the Abeta amyloid protofibril, has been identified. Here, we report studies of Abeta amyloid protofibril and its seeded transition into amyloid fibrils using atomic force microscopy. Seeding of the protofibril-to-fibril transition was observed. Preformed fibrils, but not protofibrils, effectively seeded this transition. The assembly state of Abeta influenced the rate of seeded growth, indicating that protofibrils are fibril assembly precursors. The handedness of the helical surface morphology of fibrils depended on the chirality of Abeta. Finally, branched and partially wound fibrils were observed....Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E MaggioP W Mantyh
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T MiyakawaS Katsuragi
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A RoherD KuKuruga
Mar 1, 1995·Neurodegeneration : a Journal for Neurodegenerative Disorders, Neuroprotection, and Neuroregeneration·D R HowlettG W Roberts
Jun 1, 1997·Journal of Structural Biology·C S GoldsburyJ Kistler

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Citations

Dec 31, 2004·Analytical and Bioanalytical Chemistry·Igor K LednevMing Xu
Mar 27, 2013·Analytical and Bioanalytical Chemistry·Anthony Veloso, Kagan Kerman
May 12, 2004·Biochimica Et Biophysica Acta·Vladimir N Uversky, Anthony L Fink
May 9, 2013·Brain Research·Maximilian GahrCarlos Schönfeldt-Lecuona
Oct 30, 2003·Journal of Molecular Biology·Rekha SrinivasanMichael G Zagorski
Nov 5, 2003·Journal of Molecular Biology·Patrizia Polverino de LauretoAngelo Fontana
Dec 31, 2003·Journal of Molecular Biology·Justin LegleiterTomasz Kowalewski
Dec 11, 2002·Journal of Molecular Biology·A J ModlerG Damaschun
Jul 10, 2003·Journal of Molecular Biology·Neil M KadNeil H Thomson
Jun 20, 2003·Neuropharmacology·Kumlesh K DevHerman van der Putten
Feb 19, 2000·Current Opinion in Structural Biology·J C Rochet, P T Lansbury
Dec 12, 2002·Micron : the International Research and Review Journal for Microscopy·J R Harris
Sep 28, 2000·Current Opinion in Chemical Biology·R Tycko
Feb 1, 1999·Analytical Chemistry
Jan 3, 2007·Biochemistry·Ronald WetzelAngela D Williams
Aug 4, 2012·ACS Chemical Neuroscience·Geeta S ParanjapeMichael R Nichols
Feb 9, 2006·Journal of the American Chemical Society·Hung D Nguyen, Carol K Hall
Jan 31, 2012·Nature Neuroscience·Iryna BenilovaBart De Strooper
Nov 16, 2004·Nature Structural & Molecular Biology·Jennifer L WackerPaul J Muchowski
Apr 2, 1999·Scandinavian Journal of Immunology·M NyboE Holm Nielsen
Jun 30, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rajaraman KrishnanSusan Lindquist
Feb 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shuai ZhangMingdong Dong
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·E H KooJ W Kelly
Mar 2, 2011·The Journal of Biological Chemistry·Driss El MoustaineJoan Torrent
Aug 15, 2012·The Journal of Biological Chemistry·Yizhou ZhouMatthew R Chapman
Oct 24, 2009·Protein Engineering, Design & Selection : PEDS·Dave E DunstanJoseph Bertolini
Nov 21, 2001·Protein Science : a Publication of the Protein Society·F ChitiM Stefani
Jul 13, 2000·Protein Science : a Publication of the Protein Society·M AltmanS Zhang
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Apr 26, 2005·Microbial Cell Factories·Salvador Ventura
Nov 15, 2008·PLoS Computational Biology·Stefan AuerMichele Vendruscolo

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