Atomic force microscopy analysis of enveloped and non-enveloped viral entry into, and egress from, cultured cells

Ultramicroscopy
M MoloneyH O'Shea

Abstract

Since its invention, the atomic force microscope has been used to image a wide variety of biological samples, including viruses. Viral entry into, and egress from, cultured cells has been extensively studied using numerous scientific techniques and to a limited extent using atomic force microscopy. One of the main structural differences that can exist between viruses is the absence, or presence, of an envelope and this factor has consequences for the mode of viral entry and egress. In this study, the entry into, and egress from, cultured cells of enveloped and non-enveloped viruses were investigated using atomic force microscopy. No significant cell surface changes were observed following infection with enveloped or non-enveloped viruses. Although roughness analysis of viral entry revealed cell smoothing post-infection, no differences between the roughness values of enveloped and non-enveloped viral entry were observed. Line analysis of viral entry revealed minor differences between cells infected with an enveloped rather than a non-enveloped virus. These differences may represent a distinction between the uptake processes of enveloped and non-enveloped viruses. Studies of viral egress revealed that infected cells were undergoi...Continue Reading

References

Jul 1, 1992·Ultramicroscopy·W HäberleG Binnig
Oct 1, 1990·Journal of Structural Biology·H J ButtP K Hansma
Jul 1, 1997·Journal of Structural Biology·U G HofmannM Radmacher
Oct 23, 1997·Biophysical Journal·F M OhnesorgeG Binnig
Nov 5, 1997·Current Opinion in Structural Biology·C BustamanteD J Keller
Apr 17, 1998·Biophysical Journal·T H KellerD Klenerman
Apr 29, 1998·FEBS Letters·Y F DryginI V Yaminsky
Jul 24, 1998·Biophysical Journal·C Le GrimellecJ P Goudonnet
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber
Sep 5, 2002·Ultramicroscopy·M MoloneyH O'Shea

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Citations

Jun 20, 2019·Scanning·Andreas StylianouEleni Alexandratou
Aug 6, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Ruana Cardoso-LimaLuciana M Rebelo Alencar

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