Abstract
The atomic force microscope (AFM) has been used to image a wide variety of biological samples, including cultured cells, in air. Whilst cultured cells have been prepared for AFM analysis using a variety of matrices and fixatives, a definitive study of sample preparation and its effects on cell morphology has not, as far as the authors are aware, previously been reported. Although a considerable number of cell fixatives exist, no single fixative is ideal for all investigations. Prior to the performance of specialised techniques, such as atomic force microscopy of cultured cells in air, the cell fixation method must be investigated and optimised. The fixative abilities of 2% paraformaldehyde-lysine-periodate, 0.25% glutaraldehyde, paraformaldehyde-glutaraldehyde, 4% phosphate-buffered formal saline, 1% formaldehyde, methanol:acetone, formal saline, 4% paraformaldehyde and ethanol:acetic acid were assessed in this study. A qualitative assessment system was used to evaluate the efficacy of the above fixatives using conventional fixation criteria (i.e. the presence of fibroblastic morphology consistent with optical microscopy and the absence of fixation artifacts). The optimal fixative was identified as 4% paraformaldehyde, which wa...Continue Reading
References
Jul 1, 1992·Ultramicroscopy·W HäberleG Binnig
Dec 1, 1994·Journal of Microscopy·D Ricci, M Grattarola
Jan 1, 1996·Journal of Microscopy·F BraetE Wisse
May 1, 1996·Journal of Microscopy·P G HaydonS P Marchese-Ragona
Jun 1, 1996·Journal of Microscopy·W H KalleA K Raap
Apr 1, 1997·Journal of Microscopy·F BraetE Wisse
Jul 1, 1997·Journal of Structural Biology·U G HofmannM Radmacher
Mar 21, 1998·Journal of Microscopy·B WeynW Jacob
Jul 23, 1998·Journal of Microscopy·F BraetE Wisse
Jul 24, 1998·Biophysical Journal·C Le GrimellecJ P Goudonnet
Nov 18, 1998·Current Opinion in Chemical Biology·H G Hansma, L Pietrasanta
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber
Citations
Jun 24, 2008·Biomechanics and Modeling in Mechanobiology·Mark A Van DoormaalC Ross Ethier
Sep 2, 2011·Applied Microbiology and Biotechnology·Yuanqing Chao, Tong Zhang
Nov 23, 2006·European Biophysics Journal : EBJ·Zoltán BálintGyörgy Váró
May 1, 2008·Stem Cells and Development·Serena BarachiniMario Petrini
Jul 3, 2004·Ultramicroscopy·M MoloneyH O'Shea
Jan 16, 2013·Analytical and Bioanalytical Chemistry·Li-Fang ChiuDar-Bin Shieh
Aug 27, 2010·Micron : the International Research and Review Journal for Microscopy·N AndreevaG Ferrini
Nov 28, 2009·Micron : the International Research and Review Journal for Microscopy·Gi-Ja LeeHun-Kuk Park
Jul 15, 2009·Micron : the International Research and Review Journal for Microscopy·Michal Jerzy WozniakGuoping Chen
Oct 1, 2008·Nanomedicine : Nanotechnology, Biology, and Medicine·Anna A BirukovaKonstantin G Birukov
Jul 16, 2014·Biochimica Et Biophysica Acta·E StefanuttiG Risuleo
May 15, 2015·Scientific Reports·A GianoncelliM Kiskinova
May 5, 2007·Macromolecular Bioscience·Serena DantiAndrea Pietrabissa
Feb 27, 2007·Microscopy Research and Technique·Mirella FalconiGiovanni Mazzotti
Jan 6, 2007·Journal of Microscopy·Hiroshi MuramatsuJong Min Kim
Oct 16, 2010·Journal of Microscopy·L W FrancisC J Wright
Sep 8, 2011·Scanning·Bao You LiuHeng Chen
Mar 20, 2015·Cytoskeleton·Marta Targosz-KoreckaMarek Szymonski
Jan 9, 2010·Journal of Microscopy·B StädlerA Franco-Obregón
Apr 15, 2008·Biophysical Journal·Fernando Terán ArceSteven M Dudek
May 12, 2005·Acta histochemica·Agnieszka PernakJuliusz Pernak
Jul 21, 2015·Journal of Virological Methods·Mia KumarMichael R Holbrook
Oct 2, 2012·Neurochemistry International·Guillermo BodegaBenjamín Fernández
Dec 30, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Devanesan Arul AnanthThilagar Sivasudha
Mar 25, 2015·PloS One·Nina RecekJonathan Sherman
Jan 13, 2006·Colloids and Surfaces. B, Biointerfaces·Cathy E McNameeKo Higashitani
Sep 20, 2017·Environmental Microbiology Reports·Venkata G PoosarlaThomas K Wood
Dec 7, 2017·PloS One·Elizabeth R CattaneoMaria R Gonzalez-Baro
Nov 15, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Katerina CizkovaZdenek Tauber
Sep 14, 2020·Microbiome·Bastian SeelbinderGianni Panagiotou