Atomic force microscopy of BHK-21 cells: an investigation of cell fixation techniques

Ultramicroscopy
M MoloneyH O'Shea

Abstract

The atomic force microscope (AFM) has been used to image a wide variety of biological samples, including cultured cells, in air. Whilst cultured cells have been prepared for AFM analysis using a variety of matrices and fixatives, a definitive study of sample preparation and its effects on cell morphology has not, as far as the authors are aware, previously been reported. Although a considerable number of cell fixatives exist, no single fixative is ideal for all investigations. Prior to the performance of specialised techniques, such as atomic force microscopy of cultured cells in air, the cell fixation method must be investigated and optimised. The fixative abilities of 2% paraformaldehyde-lysine-periodate, 0.25% glutaraldehyde, paraformaldehyde-glutaraldehyde, 4% phosphate-buffered formal saline, 1% formaldehyde, methanol:acetone, formal saline, 4% paraformaldehyde and ethanol:acetic acid were assessed in this study. A qualitative assessment system was used to evaluate the efficacy of the above fixatives using conventional fixation criteria (i.e. the presence of fibroblastic morphology consistent with optical microscopy and the absence of fixation artifacts). The optimal fixative was identified as 4% paraformaldehyde, which wa...Continue Reading

References

Jul 1, 1992·Ultramicroscopy·W HäberleG Binnig
May 1, 1996·Journal of Microscopy·P G HaydonS P Marchese-Ragona
Jun 1, 1996·Journal of Microscopy·W H KalleA K Raap
Jul 1, 1997·Journal of Structural Biology·U G HofmannM Radmacher
Jul 24, 1998·Biophysical Journal·C Le GrimellecJ P Goudonnet
Nov 18, 1998·Current Opinion in Chemical Biology·H G Hansma, L Pietrasanta
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber

❮ Previous
Next ❯

Citations

Jun 24, 2008·Biomechanics and Modeling in Mechanobiology·Mark A Van DoormaalC Ross Ethier
Nov 23, 2006·European Biophysics Journal : EBJ·Zoltán BálintGyörgy Váró
Jan 16, 2013·Analytical and Bioanalytical Chemistry·Li-Fang ChiuDar-Bin Shieh
Aug 27, 2010·Micron : the International Research and Review Journal for Microscopy·N AndreevaG Ferrini
Nov 28, 2009·Micron : the International Research and Review Journal for Microscopy·Gi-Ja LeeHun-Kuk Park
Jul 15, 2009·Micron : the International Research and Review Journal for Microscopy·Michal Jerzy WozniakGuoping Chen
Feb 27, 2007·Microscopy Research and Technique·Mirella FalconiGiovanni Mazzotti
Jan 6, 2007·Journal of Microscopy·Hiroshi MuramatsuJong Min Kim
Jan 9, 2010·Journal of Microscopy·B StädlerA Franco-Obregón
May 12, 2005·Acta histochemica·Agnieszka PernakJuliusz Pernak
Jul 21, 2015·Journal of Virological Methods·Mia KumarMichael R Holbrook
Oct 2, 2012·Neurochemistry International·Guillermo BodegaBenjamín Fernández
Dec 30, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Devanesan Arul AnanthThilagar Sivasudha
Jan 13, 2006·Colloids and Surfaces. B, Biointerfaces·Cathy E McNameeKo Higashitani
Nov 15, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Katerina CizkovaZdenek Tauber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.