PMID: 7932654Jun 1, 1994Paper

Atomic force microscopy of peritoneal macrophages after particle phagocytosis

The Journal of Membrane Biology
M BeckmannF Lang

Abstract

The atomic force microscope was used to image peritoneal macrophages after phagocytosis of latex beads with 0.45 microns in diameter and of zymosan particles. The rigidity of the phagocytosed material allowed to image the live membrane at forces below 2 nN. Repeated scanning of the membrane unavoidably caused the protrusion of the beads and increased their virtual height. The influence of fixation by glutaraldehyde on the image and the corresponding force vs. distance curves were analyzed and compared. Short treatment with Triton X-100 enabled us to identify intracellular components, such as embedded latex beads, cell nucleus and cytoskeletal strands. The data demonstrate that it is possible to image living cells if they are bolstered by stiff material.

Citations

Nov 20, 2004·Emerging Infectious Diseases·M L NgE E Ooi
Dec 3, 2013·Biochimica Et Biophysica Acta·Flavien PilletEtienne Dague
Apr 3, 2012·Micron : the International Research and Review Journal for Microscopy·F Braet, E Wisse
Mar 1, 1997·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·M Radmacher
Jun 23, 2020·Colloids and Surfaces. B, Biointerfaces·Thomas J DaubenKlaus D Jandt

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