Abstract
Hyperthermophilic glycoside hydrolase family 12 endocellulase (EGPf) from the archaeon Pyrococcus furiosus catalyzes the hydrolytic cleavage of β-1,4-glucosidic linkage in β-glucan cellulose. A truncated EGPf (EGPfΔN30) mutant lacking the proline and hydroxyl-residue rich region at the N terminus was constructed, and its crystal structure was resolved at an atomic resolution of 1.07 Å. Our results indicate that the structure of EGPf, which consists of a β-jelly roll, exhibits structural similarity with the endocellulase of Thermotoga maritima. Additionally, we further determined that the thermostability of EGPf is maintained in part by the binding of Ca²⁺ in a DxDxDG Ca²⁺-binding motif, atypical of most archaeal proteins.
References
Jun 1, 1996·Journal of Computer-aided Molecular Design·D M van AaltenG Vriend
Jul 1, 1997·Nature Structural Biology·R H Kretsinger
Dec 29, 1998·Journal of Bacteriology·M W BauerR M Kelly
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt, T A Jones
Jun 21, 2001·Applied Microbiology and Biotechnology·K B WicherO Holst
Apr 27, 2002·Acta Crystallographica. Section D, Biological Crystallography·Marjorie M Harding
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Sep 18, 2004·Extremophiles : Life Under Extreme Conditions·Yasuhiro KashimaKazuhiko Ishikawa
Oct 13, 2004·Journal of Molecular Biology·Daniel J Rigden, Michael Y Galperin
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jun 14, 2005·Progress in Biophysics and Molecular Biology·Mats SandgrenColin Mitchinson
Dec 21, 2007·Acta Crystallographica. Section D, Biological Crystallography·Fei LongGarib N Murshudov
Oct 23, 2009·Proteins·Han-Woo Kim, Kazuhiko Ishikawa
May 12, 2011·The Biochemical Journal·Han-Woo Kim, Kazuhiko Ishikawa
Jul 2, 2011·PloS One·Daniel J RigdenMichael Y Galperin
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor
Citations
Oct 28, 2015·International Journal of Molecular Sciences·Junling WangZuoming Zhang
Apr 30, 2014·Advances in Bioinformatics·Tatyana Aleksandrovna Khrustaleva
Nov 13, 2014·Journal of Industrial Microbiology & Biotechnology·Seiichiro KishishitaKazuhiko Ishikawa
Sep 12, 2014·Bioscience, Biotechnology, and Biochemistry·Misumi Kataoka, Kazuhiko Ishikawa
May 21, 2016·Frontiers in Microbiology·Sergey N GavrilovBettina Siebers
Nov 13, 2015·PloS One·Huaidong ZhangYanhe Ma
Jul 10, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Misumi Kataoka, Kazuhiko Ishikawa
Jul 31, 2019·Molecular Biology Reports·Zhu ZhuYuqi Qin
Oct 7, 2015·Frontiers in Microbiology·Hui YuanXi-Peng Liu
Jan 30, 2015·Chemical Reviews·Christina M PayneGregg T Beckham