Atomic resolution structure of the double mutant (K53,56M) of bovine pancreatic phospholipase A2

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
K SekarM D Tsai

Abstract

The structure of the double mutant K53,56M has previously been refined at 1.9 A resolution using room-temperature data. The present paper reports the crystal structure of the same mutant K53,56M refined against 1.1 A data collected using synchrotron radiation. A total of 116 main-chain atoms from 29 residues and 44 side chains are modelled in alternate conformations. Most of the interfacial binding residues are found to be disordered and alternate conformations could be recognized. The second calcium ion-binding site residue Glu92 adopts two alternate conformations. The minor and major conformations of Glu92 correspond to the second calcium ion bound and unbound states.

References

Jan 1, 1976·Annual Review of Biophysics and Bioengineering·R Verger
Jan 1, 1991·Proteins·F Ramirez, M K Jain
Jan 1, 1997·Trends in Biochemical Sciences·E A Dennis
Oct 8, 1998·Acta Crystallographica. Section D, Biological Crystallography·K SekarM Sundaralingam
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·K Sekar, M Sundaralingam
Jul 27, 1999·Acta Crystallographica. Section D, Biological Crystallography·M M Harding
Mar 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·R A SteinerB W Dijkstra
Jul 1, 1963·Journal of Molecular Biology·G N RAMACHANDRANV SASISEKHARAN
Oct 22, 1963·Biochimica Et Biophysica Acta· VAN DEENENL, G H DE HAAS
Oct 8, 2003·Journal of Molecular Biology·K SekarM-D Tsai
Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·K SekarM-D Tsai

❮ Previous
Next ❯

Citations

Jan 15, 2014·Acta Crystallographica. Section D, Biological Crystallography·Manickam YogavelAmit Sharma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section D, Biological Crystallography
M A WalshK S Wilson
Acta Crystallographica. Section D, Biological Crystallography
Jozef SevcíkKeith S Wilson
Acta Crystallographica. Section D, Biological Crystallography
A AddlagattaM Jaskolski
© 2021 Meta ULC. All rights reserved