Atomic structure of the ectodomain from HIV-1 gp41

Nature
W WeissenhornD C Wiley

Abstract

Fusion of viral and cellular membranes by the envelope glycoprotein gp120/gp41 effects entry of HIV-1 into the cell. The precursor, gp160, is cleaved post-translationally into gp120 and gp41 which remain non-covalently associated. Binding to both CD4 and a co-receptor leads to the conformational changes in gp120/gp41 needed for membrane fusion. We used X-ray crystallography to determine the structure of the protease-resistant part of a gp41 ectodomain solubilized with a trimeric GCN4 coiled coil in place of the amino-terminal fusion peptide. The core of the molecule is found to be an extended, triple-stranded alpha-helical coiled coil with the amino terminus at its tip. A carboxy-terminal alpha-helix packs in the reverse direction against the outside of the coiled coil, placing the amino and carboxy termini near each other at one end of the long rod. These features, and the existence of a similar reversal of chain direction in the fusion pH-induced conformation of influenza virus HA2 and in the transmembrane subunit of Moloney murine leukaemia virus (Fig. 1a-d), suggest a common mechanism for initiating fusion.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C WildT Matthews
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T K HartP J Bugelski
Nov 23, 1990·Science·J P MooreQ J Sattentau
Aug 1, 1989·AIDS Research and Human Retroviruses·W R GallaherR C Montelaro
Nov 1, 1988·The Journal of General Virology·R W RuigrokD C Wiley
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·C T WildT J Matthews
Sep 1, 1994·Nature·P B HarburyT Alber
Jan 1, 1993·Annual Review of Physical Chemistry·S LeikinR P Rand
May 1, 1996·Nature Structural Biology·D FassP S Kim
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·J ChenD C Wiley
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·J J SkehelD C Wiley
Dec 1, 1995·Nature Structural Biology·M LuP S Kim
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·W WeissenhornD C Wiley
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

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Citations

Aug 24, 1999·European Journal of Immunology·G W LynchA L Cunningham
Jul 31, 1998·Protein Science : a Publication of the Protein Society·R J CenterP Poumbourios
Nov 25, 1998·Protein Science : a Publication of the Protein Society·C Gray, L K Tamm
Jan 25, 2011·European Biophysics Journal : EBJ·Avraham Ashkenazi, Yechiel Shai
Aug 5, 2011·Archives of Virology·Salvatore DimonteMuhammed Babakir-Mina
May 7, 2010·Protoplasma·Jennifer A ThorleyJoshua Zachary Rappoport
Sep 11, 2012·Amino Acids·Yu BaiKeliang Liu
Sep 16, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Thomas DangeDavid Grünwald
Jul 31, 2013·Journal of Computer-aided Molecular Design·Miriam GochinDale L Boger
Feb 15, 2001·Current Infectious Disease Reports·Georgia D. Tomaras, Michael L. Greenberg
May 29, 2008·Immunologic Research·Soichi HaraguchiNoorbibi K Day-Good
Apr 1, 2009·Virologica Sinica·Haiqing JiangLijun Rong
Apr 2, 2013·Antiviral Research·Aiman A Haqqani, John C Tilton
Dec 4, 2003·Biochemical and Biophysical Research Communications·Gadi BorkowAviva Lapidot
May 26, 2005·Biochemical and Biophysical Research Communications·Ling NiPo Tien
Apr 22, 2004·Journal of Immunological Methods·David OpalkaMark T Esser
Jan 27, 2004·Journal of Molecular Biology·Daniel N MartiIlian Jelesarov
Jun 11, 2005·Journal of Molecular Biology·Yongping PanRuth Nussinov
Jul 16, 2013·Trends in Microbiology·Lesley A EarlSriram Subramaniam
Feb 13, 2001·Immunology Letters·X N DongY H Chen
Jan 16, 2003·Immunology Letters·Theodore C Pierson, Robert W Doms
Apr 15, 1999·Immunology Letters·Q J SattentauS Ugolini
Nov 24, 1999·Immunology Today·Y H ChenM P Dierich
Aug 25, 2001·Virus Research·I Le BlancM C Dokhélar
Apr 11, 2003·Virus Research·Gary J Nabel
Jun 6, 2000·Advanced Drug Delivery Reviews·I MartinR M Epand

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