Atorvastatin effects on SREBF1a and SCAP gene expression in mononuclear cells and its relation with lowering-lipids response

Clinica Chimica Acta; International Journal of Clinical Chemistry
Simone Sorkin AraziRosario Dominguez Crespo Hirata

Abstract

The transcription factors SREBP1 and SCAP are involved in intracellular cholesterol homeostasis. Polymorphisms of these genes have been associated with variations on serum lipid levels and response to statins that are potent cholesterol-lowering drugs. We evaluated the effects of atorvastatin on SREBF1a and SCAP mRNA expression in peripheral blood mononuclear cells (PBMC) and a possible association with gene polymorphisms and lowering-cholesterol response. Fifty-nine hypercholesterolemic patients were treated with atorvastatin (10 mg/day for 4 weeks). Serum lipid profile and mRNA expression in PBMC were assessed before and after the treatment. Gene expression was quantified by real-time PCR using GAPD as endogenous reference and mRNA expression in HepG2 cells as calibrator. SREBF1 -36delG and SCAP A2386G polymorphisms were detected by PCR-RFLP. Our results showed that transcription of SREBF1a and SCAP was coordinately regulated by atorvastatin (r=0.595, p<0.001), and that reduction in SCAP transcription was associated with the 2386AA genotype (p=0.019). Individuals who responded to atorvastatin with a downregulation of SCAP had also a lower triglyceride compared to those who responded to atorvastatin with an upregulation of SCA...Continue Reading

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Citations

Sep 29, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hong-Hee WonSoo-Youn Lee
Jun 18, 2009·Pharmacogenomics·Fabiana D V GenvigirRosario D C Hirata
Jun 18, 2009·Pharmacogenomics·Maria Alice Vieira WillrichRosario Dominguez Crespo Hirata
Nov 2, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·André Ducati LuchessiMario Hiroyuki Hirata
Mar 12, 2015·The Cochrane Database of Systematic Reviews·Stephen P AdamsJames M Wright
Aug 18, 2021·Pharmacological Reports : PR·Carolina Dagli-HernandezRosario Dominguez Crespo Hirata

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