Atorvastatin inhibits cholesterol-induced caspase-3 cleavage through down-regulation of p38 and up-regulation of Bcl-2 in the rat carotid artery

Cardiovascular Journal of Africa
Roshanak BayatmakooPouran Karimi

Abstract

Atherosclerotic lesions in the carotid arteries lead to a broad range of cerebrovascular disorders such as vascular dementia and ischaemic stroke. Recent studies have verified the beneficial role of atorvastatin (AV) in atherosclerosis. Despite a large body of studies, the mechanisms underlying this effect have not been completely explained. In this study, several experiments were performed on atherosclerotic rat models to investigate the anti-inflammatory and anti-apoptotic effect of AV in the carotid artery. In this experimental study, 40 male Wistar rats (250 ± 25 g) were randomly divided into four groups: rats on a normal diet (ND; n = 10); a high-cholesterol diet (HD; n = 10); a high-cholesterol diet plus AV (HD + AV; n = 10) ; and the AV control group (AV; n = 10). Cleavage of caspase-3 protein, expression of B-cell lymphoma 2 (Bcl-2) as well as phosphorylation of p38 mitogen-activated protein kinase (MAPK) were determined by immunoblotting assay in the carotid artery homogenate. Plasma atherogenic indices, including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured by colorimetric assay at the end of the experiment. Plasma levels of oxidised...Continue Reading

Citations

Jul 4, 2021·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Xingyang YiShaozhi Bao
Aug 24, 2021·Frontiers in Neuroscience·Ya-Ting HuangShu-Long Yang
Oct 8, 2020·Cardiology in Review·Bhawneet Chadha, William H Frishman

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Methods Mentioned

BETA
ELISA
protein folding

Software Mentioned

Vision Work LS

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